徳島大学 教育・研究者情報データベース(EDB)

1. (英) Arinawati Dian Yosi / (日) Dian Yosi Arinawati / (読) でぃあん よし ありなわてぃ

   役割	任意
   貢献度	任意
   学籍番号	推奨	****

2. 三好 圭子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. 谷村 綾子

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 堀口 大吾([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. (英) Hagita Hiroko

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. 野間 隆文

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Deciphering defective amelogenesis using invitro culture systems.

(日) The conventional two-dimensional (2D) in vitro culture system is frequently used to analyze the gene expression with or without extracellular signals. However, the cells derived from primary culture and cell lines frequently deviate the gene expression profile compared to the corresponding in vivo samples, which sometimes misleads the actual gene regulation in vivo. To overcome this gap, we developed the comparative 2D and 3D in vitro culture systems and applied them to the genetic study of amelogenesis imperfecta (AI) as a model. Recently, we found specificity protein 6 (Sp6) mutation in an autosomal-recessive AI rat that was previously named AMI. We constructed 3D structure of ARE-B30 cells (AMI-derived rat dental epithelial cells) or G5 (control wild type cells) combined with RPC-C2A cells (rat pulp cell line) separated by the collagen membrane, while in 2D structure, ARE-B30 or G5 was cultured with or without the collagen membrane. Comparative analysis of amelogenesis-related gene expression in ARE-B30 and G5 using our 2D and 3D in vitro systems revealed distinct expression profiles, showing the causative outcomes. Bone morphogenetic protein 2 and follistatin were reciprocally expressed in G5, but not in ARE-B30 cells. All-or-none expression of amelotin, kallikrein-related peptidase 4, and nerve growth factor receptor was observed in both cell types. In conclusion, our in vitro culture systems detected the phenotypical differences in the expression of the stage-specific amelogenesis-related genes. Parallel analysis with 2D and 3D culture systems may provide a platform to understand the molecular basis for defective amelogenesis caused by Sp6 mutation.

1. (英) PublicationType: Journal Article