徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.総合科学部.社会創生学科.環境共生コース(2009年4月1日〜2024年3月31日)
2. 徳島大学.生物資源産業学部.生物資源産業学科.食料科学コース(2016年4月1日〜)

1. (英) Wada Honoka

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2. (英) Oyama Keisuke

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3. (英) Kamae Risa

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4. 増田 俊哉

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5. 金丸 芳

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6. 横井川 久己男

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7. 小山 保夫

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(英) Zinc-dependent and independent actions of hydroxyhydroquinone on rat thymic lymphocytes

(英) Coffee contains hydroxyhydroquinone (HHQ). HHQ is one of by-products released during bean roasting. Therefore, it is important to elucidate the bioactivity of HHQ to predict its beneficial or adverse effects on humans. We studied zinc-dependent and independent actions of commercially-procured synthetic HHQ in rat thymocytes using flow cytometric techniques with propidium iodide, FluoZin-3-AM, 5-chloromethylfluorescein diacetate, and annexin V-FITC. HHQ at 10¬50 µM elevated intracellular Zn2+ levels by releasing intracellular Zn2+. HHQ at 10 µM increased cellular thiol content in a Zinc-dependent manner. However, HHQ at 30 50 µM reduced cellular thiol content. Although the latter actions of HHQ (30 50 µM) were suggested to increase cell vulnerability to oxidative stress, HHQ at 0.3 100 µM significantly protected cells against oxidative stress induced by H2O2. The process of cell death induced by H2O2 was delayed by HHQ, although both H2O2 and HHQ increased the population of annexin V-positive living cells. However, HHQ at 10 30 µM promoted cell death induced by A23187, a calcium ionophore. HHQ at 10 30 µM exerted contrasting effects on cell death caused by oxidative stress and Ca2+ overload. Because HHQ is considered to possess diverse cellular actions, coffee with reduced amount of HHQ may be preferable to avoid potential adverse effects.

1. 亜鉛(zinc)
2. (英) Hydroyxhydroquinone