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著作: Srinivasan Dinesh/[藤野 裕道]/Regan John W/Differential internalization of the prostaglandin f(2alpha) receptor isoforms: role of protein kinase C and clathrin./[The Journal of Pharmacology and Experimental Therapeutics]

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EID
329474
EOID
865407
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LastModified
2017年8月17日(木) 16:52:53
Operator
藤野 裕道
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藤野 裕道
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Srinivasan Dinesh
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 藤野 裕道([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.生命薬理学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Regan John W
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Differential internalization of the prostaglandin f(2alpha) receptor isoforms: role of protein kinase C and clathrin.

副題 任意
要約 任意

(英) FP prostanoid receptors are G-protein-coupled receptors that mediate the actions of prostaglandin F(2alpha) (PGF(2alpha)). Alternative mRNA splicing gives rise to two isoforms, FP(A) and FP(B), which are identical except for their intracellular carboxyl termini. In this study, we examined the internalization of recombinant FLAG-epitope-tagged FP(A) and FP(B) receptors that were stably expressed in human embryonic kidney-293 cells. Cell surface receptors on live cells were labeled with anti-FLAG antibodies either in the presence or absence of PGF(2alpha) and were examined by immunofluorescence microscopy. In the absence of PGF(2alpha), FP(A)-expressing cells were labeled predominantly on the cell surface; however, FP(B)-expressing cells were labeled on both the cell surface and intracellularly, indicating constitutive internalization of the FP(B) isoform. After treatment with PGF(2alpha), FP(A)-expressing cells were labeled intracellularly, reflecting receptor internalization, which could be mimicked with phorbol 12-myristyl 13-acetate (PMA), an activator of protein kinase C (PKC). Pretreatment of FP(A)-expressing cells with Gö 6976 [12-(2-cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo[2,3-a]pyrrolo[3,4-c]carbozole], an inhibitor of PKC, blocked both PGF(2alpha)- and PMA-induced receptor internalization. However, Gö 6976 did not block constitutive internalization of the FP(B) isoform, suggesting that the mechanisms of receptor internalization differ between the FP(A) and FP(B) isoforms. Furthermore, pretreatment with sucrose, an inhibitor of clathrin-dependent internalization, blocked PGF(2alpha)-induced internalization of the FP(A) isoform but did not block constitutive internalization of the FP(B) isoform. In conclusion, the FP(A) receptor isoform shows an agonist-induced internalization involving PKC and clathrin, whereas the FP(B) isoform undergoes agonist-independent internalization that does not involve PKC or clathrin.

キーワード 推奨
  1. (英) Cell Line
  2. (英) Clathrin
  3. (英) Dinoprost
  4. (英) Enzyme Activation
  5. (英) Epitopes
  6. (英) Humans
  7. (英) Immunoblotting
  8. (英) Isomerism
  9. (英) Microscopy, Fluorescence
  10. (英) Phosphorylation
  11. (英) Prostaglandin Antagonists
  12. (英) Protein Kinase C
  13. (英) Receptors, Prostaglandin
  14. (英) Sucrose
  15. (英) Tetradecanoylphorbol Acetate
  16. (英) Tyrosine
発行所 推奨
誌名 必須 The Journal of Pharmacology and Experimental Therapeutics(American Society for Pharmacology and Experimental Therapeutics)
(pISSN: 0022-3565, eISSN: 1521-0103)
ISSN 任意 0022-3565
ISSN: 0022-3565 (pISSN: 0022-3565, eISSN: 1521-0103)
Title: The Journal of pharmacology and experimental therapeutics
Title(ISO): J Pharmacol Exp Ther
Publisher: American Society for Pharmacology and Experimental Therapeutics
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 302
必須 1
必須 219 224
都市 任意
年月日 必須 2002年 7月 初日
URL 任意
DOI 任意
PMID 任意 12065720    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article