徳島大学 教育・研究者情報データベース(EDB)

1. (英) Hosokawa Yuki

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2. 平尾 功治

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3. 湯本 浩通([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])

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4. (英) Washio Ayako

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5. 中西 正([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.再生歯科治療学]/[徳島大学.病院.診療科.歯科.むし歯科(第一保存科)])

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6. 武川 大輔([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.再生歯科治療学]/[徳島大学.病院.診療科.歯科.むし歯科(第一保存科)])

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7. (英) Kitamura Chiaki

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8. 松尾 敬志

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(英) Functional roles of NOD1 in odontoblasts on dental pulp innate immunity

(英) Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.

1. (英) Animals
2. (英) Cell Line
3. 細胞質分裂(cytokinesis)
4. (英) Dental Pulp
5. 自然免疫(innate immunity)
6. (英) Inflammation Mediators
7. (英) Nod1 Signaling Adaptor Protein
8. (英) Odontoblasts
9. (英) Rats

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) CoiStatement: The authors declare that there is no conflict of interest regarding the publication of this paper.