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著作: [西川 泰史]/[梶浦 由加里]/Lew Jung Hwan/[木戸 淳一]/[永田 俊彦]/[成石 浩司]/Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts./[Journal of Cellular Physiology]

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EID
326016
EOID
949335
Map
0
LastModified
2019年7月8日(月) 11:05:55
Operator
三木 ちひろ
Avail
TRUE
Censor
0
Owner
成石 浩司
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 西川 泰史([徳島大学.病院.中央診療施設等.口腔インプラントセンター])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 梶浦 由加里
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Lew Jung Hwan
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 木戸 淳一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 永田 俊彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 成石 浩司
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Calprotectin Induces IL-6 and MCP-1 Production via Toll-Like Receptor 4 Signaling in Human Gingival Fibroblasts.

副題 任意
要約 任意

(英) Calprotectin, a heterodimer of S100A8 and S100A9 molecules, is associated with inflammatory diseases such as inflammatory bowel disease. We have reported that calprotectin levels in gingival crevicular fluids of periodontitis patients are significantly higher than in healthy subjects. However, the functions of calprotectin in pathophysiology of periodontitis are still unknown. The aim of this study is to investigate the effects of calprotectin on the productivity of inflammatory cytokines in human gingival fibroblasts (HGFs). The HGFs cell line CRL-2014® (ATCC) were cultured, and total RNAs were collected to examine the expression of TLR2/4 and RAGE mRNA using RT-PCR. After the cells were treated with S100A8, S100A9, and calprotectin, supernatants were collected and the levels of IL-6 and MCP-1 were measured using ELISA methods. To examine the intracellular signals involved in calprotectin-induced cytokine production, several chemical inhibitors were used. Furthermore, after the siRNA-mediated TLR4 down-regulated cells were treated with S100A8, S100A9, and calprotectin, the levels of IL-6 and MCP-1 were also measured. HGFs showed greater expression of TLR4 mRNA, but not TLR2 and RAGE mRNA compared with human oral epithelial cells. Calprotectin increased significantly the production of MCP-1 and IL-6 in HGFs, and the cytokine productions were significantly suppressed in the cells treated with MAPKs, NF-B, and TLR4 inhibitors. Furthermore, calprotectin-mediated MCP-1 and IL-6 production were significantly suppressed in TLR4 down-regulated cells. Taken together, calprotectin induces IL-6 and MCP-1 production in HGFs via TLR4 signaling that involves MAPK and NF-B, resulting in the progression of periodontitis. J. Cell. Physiol. 232: 1862-1871, 2017. © 2016 Wiley Periodicals, Inc.

キーワード 推奨
  1. (英) Antigens, Neoplasm
  2. (英) Cell Line, Tumor
  3. 細胞増殖·分化(cell proliferation and differentiation)
  4. (英) Chemokine CCL2
  5. (英) Down-Regulation
  6. (英) Fibroblasts
  7. (英) Gene Expression Regulation
  8. (英) Gingiva
  9. (英) Humans
  10. (英) Inflammation Mediators
  11. (英) Interleukin-6
  12. (英) Intracellular Space
  13. (英) Leukocyte L1 Antigen Complex
  14. (英) Mitogen-Activated Protein Kinases
  15. (英) Models, Biological
  16. (英) NF-kappa B
  17. (英) Protein Kinase Inhibitors
  18. (英) RNA, Messenger
  19. (英) RNA, Small Interfering
  20. 炎症性サイトカイン受容体の情報伝達(Signal transduction of proinflammatory cytokine receptors)
  21. (英) Sulfonamides
  22. (英) Toll-Like Receptor 4
  23. (英) Transfection
発行所 推奨
誌名 必須 Journal of Cellular Physiology([Wiley-Liss, Inc.])
(pISSN: 0021-9541, eISSN: 1097-4652)
ISSN 任意 1097-4652
ISSN: 0021-9541 (pISSN: 0021-9541, eISSN: 1097-4652)
Title: Journal of cellular physiology
Title(ISO): J Cell Physiol
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 232
必須 7
必須 1862 1871
都市 任意
年月日 必須 2017年 1月 6日
URL 任意
DOI 任意 10.1002/jcp.25724    (→Scopusで検索)
PMID 任意 27925202    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意 2-s2.0-85008440585
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article