徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野(2017年4月1日〜)
2. 徳島大学.大学院社会産業理工学研究部.理工学域.自然科学系.化学分野(2017年4月1日〜)

1. (英) Oyama Keisuke

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2. 三好 德和([徳島大学.大学院社会産業理工学研究部.理工学域.自然科学系.化学分野]/[徳島大学.理工学部.理工学科.自然科学コース.化学講座])

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3. 小山 保夫

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(英) Cytometric analysis on cytotoxicity of 4,4 -methylenediphenyl diisocyanate, a chemical allergen, in rat thymocytes

(英) 4,4'-Methylenediphenyl diisocyanate (MDI) is a cross-linking agent. Chemical reactivity of MDI with endogenous substances such as albumin and glutathione (GSH) is assumed to be responsible for MDI toxicity. We examined the cytotoxic effect of MDI on rat thymocytes, under the condition that endogenous biological substances except for cells were nominally absent, in order to study chemico-biological interactions between MDI and cells. The treatment of 10 50 µM MDI for 3 h significantly increased the side scatter signal intensity of cytograms, without affecting forward scatter intensity. The increase in side scatter signal intensity by MDI was associated with an increase in cell lethality. The treatment of cells with 50 µM MDI for 3 h increased cell lethality without increasing the population of pre-apoptotic annexin V-positive living cells. In contrast, H2O2 at 100 µM significantly increased the population of annexin V positive living cells prior to cell death. MDI at 30 50 µM did not affect the increase in cell lethality induced by H2O2 or A23187. Simultaneous application of 50 µM GSH did not affect the cytotoxicity of 50 µM MDI. It was therefore concluded that the process of cell death induced by MDI could not be attributed to oxidative stress and intracellular Ca2+ overload, and that MDI possesses cytotoxic actions that are not significantly related to its chemical reactivity with GSH.