徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.病態予防医学講座.生理機能学(2004年4月1日〜2015年3月31日)
2. 徳島大学.医学部.医学科.病態予防医学講座.細胞生物学分野

1. (英) Hayashi Mikio / (日) 林 美樹夫 / (読) はやし みきお

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. 稲垣 明浩([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.生理学])

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. (英) Ivana Novak

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. (英) Matsuda Hiroko / (日) 松田 博子 / (読) まつだ ひろこ

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.

(英) Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl(-) channels important for anion secretion, we herein performed experiments on Capan-1, a human pancreatic duct cell line, using open-circuit Ussing chamber and gramicidin-perforated patch-clamp techniques. The luminal addition of adenosine increased the negative transepithelial potential difference (V te) in Capan-1 monolayers with a half-maximal effective concentration value of approximately 10 M, which corresponded to the value obtained on whole-cell Cl(-) currents in Capan-1 single cells. The effects of adenosine on V te, an equivalent short-circuit current (I sc), and whole-cell Cl(-) currents were inhibited by CFTRinh-172, a cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel inhibitor. The adenosine A2B receptor agonist, BAY 60-6583, increased I sc and whole-cell Cl(-) currents through CFTR Cl(-) channels, whereas the A2A receptor agonist, CGS 21680, had negligible effects. The A2B receptor antagonist, PSB 603, inhibited the response of I sc to adenosine. Immunohistochemical analysis showed that the A2A and A2B receptors colocalized with Ezrin in the luminal membranes of Capan-1 monolayers and in rat pancreatic ducts. Adenosine elicited the whole-cell Cl(-) currents in guinea pig duct cells. These results demonstrate that luminal adenosine regulates anion secretion by activating CFTR Cl(-) channels via adenosine A2B receptors on the luminal membranes of Capan-1 cells. The present study endorses that purinergic signaling is important in the regulation of pancreatic secretion.

1. アデノシン受容体(adenosine receptor)
2. (英) CFTR
3. (英) Cl channel / (日) クロライドチャネル / (読) くろらいどちゃねる
4. (英) Duct / (日) 分泌腺導管 / (読) ぶんぴせんどうかん
5. (英) Pancreas / (日) 膵臓 / (読) すいぞう

1. (英) Article.Affiliation: Medical Research Project, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't