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著作: Fukushima Y/Asano T/Katagiri H/Aihara M/Saitoh T/Anai M/[船木 真理]/Ogihara T/Inukai K/Matsuhashi N/Oka Y/Yazaki Y/Sugano K/Interaction between the two signal transduction systems of the histamine H2 receptor: desensitizing and sensitizing effects of histamine stimulation on histamine-dependent cAMP production in Chinese hamster ovary cells./[The Biochemical Journal]

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EID
309864
EOID
830955
Map
0
LastModified
2016年8月19日(金) 14:29:10
Operator
大家 隆弘
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Owner
船木 真理
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Fukushima Y
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Asano T
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    学籍番号 推奨
  3. (英) Katagiri H
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    学籍番号 推奨
  4. (英) Aihara M
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Saitoh T
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Anai M
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 船木 真理([徳島大学.病院.中央診療施設等.糖尿病対策センター])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Ogihara T
    役割 任意
    貢献度 任意
    学籍番号 推奨
  9. (英) Inukai K
    役割 任意
    貢献度 任意
    学籍番号 推奨
  10. (英) Matsuhashi N
    役割 任意
    貢献度 任意
    学籍番号 推奨
  11. (英) Oka Y
    役割 任意
    貢献度 任意
    学籍番号 推奨
  12. (英) Yazaki Y
    役割 任意
    貢献度 任意
    学籍番号 推奨
  13. (英) Sugano K
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Interaction between the two signal transduction systems of the histamine H2 receptor: desensitizing and sensitizing effects of histamine stimulation on histamine-dependent cAMP production in Chinese hamster ovary cells.

副題 任意
要約 任意

(英) The histamine H2 receptor is a member of the family of G-protein-coupled receptors and is linked to the activation of adenylate cyclase phospholipase C (PLC). In this study we examined the effects of protein kinase C (PKC) activation in Chinese hamster ovary (CHO) cells stably expressing canine histamine H2 receptors. Pretreatment with 100 nM phorbol 12-myristate 13-acetate at 37 degrees C for 15 min led to significant potentiation of histamine-dependent and forskolin-dependent cAMP production, whereas the biologically inactive phorbol ester, 4 alpha-phorbol 12, 13-didecanoate, was without effect. These potentiating effects were abolished by preincubation with 0.5 microM bisindolylmaleimide, a PKC inhibitor. Thus the activation of PKCs seems to be involved in the potentiation of cAMP production by acting on a post-receptor mechanism. Preincubation of a CHO cell line, CHO-H2R, with 10 microM histamine for 30 min had two effects. Maximal histamine-dependent cAMP production and forskolin-dependent cAMP production were potentiated by 36% and 105.2% respectively. The other effect was a desensitization of the histamine-dependent adenylate cyclase response as demonstrated by a three-fold increase in EC50. Administration of 0.5 microM bisindolylmaleimide before preincubation of CHO-H2R with 10 microM histamine did not alter the desensitizing effect on cAMP production, but did abolish the sensitizing effect. Preincubation of CHO-H2R cells with 10 nM histamine resulted in moderate potentiation, which was also abolished by bisindolylmaleimide, but not in desensitization of the histamine-dependent cAMP production. Thus these results suggest that preincubation with histamine had a sensitizing effect on cAMP production mediated by PLC and PKC activation, as well as a desensitizing effect on the H2 receptor. The former effect is dependent on the intensity of PLC and PKC signals delivered by H2 receptors. The latter effect requires a higher concentration of histamine.

キーワード 推奨
  1. (英) Animals
  2. (英) CHO Cells
  3. (英) Colforsin
  4. (英) Cricetinae
  5. (英) Cricetulus
  6. (英) Cyclic AMP
  7. (英) Dogs
  8. ヒスタミン(histamine)
  9. (英) Histamine Agonists
  10. (英) Indoles
  11. (英) Maleimides
  12. (英) Receptors, Histamine H2
  13. (英) Recombinant Proteins
  14. シグナル伝達(signal transduction)
  15. (英) Tetradecanoylphorbol Acetate
発行所 推奨
誌名 必須 The Biochemical Journal([The Biochemical Society])
(pISSN: 0264-6021, eISSN: 1470-8728)
ISSN 任意 0264-6021
ISSN: 0264-6021 (pISSN: 0264-6021, eISSN: 1470-8728)
Title: The Biochemical journal
Title(ISO): Biochem J
Publisher: Portland Press, Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 320 ( Pt 1)
必須
必須 27 32
都市 任意
年月日 必須 1996年 11月 15日
URL 任意
DOI 任意 10.1042/bj3200027    (→Scopusで検索)
PMID 任意 8947463    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article

  2. (英) PublicationType: Research Support, Non-U.S. Gov't