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著作: [宮本 理人]/Egawa Tatsuro/Oshima Rieko/Kurogi Eriko/Tomida Yosuke/Tsuchiya Koichiro/Hayashi Tatsuya/AICAR stimulation metabolome-widely mimics electrical contraction in isolated rat epitrochlearis muscle./[American Journal of Physiology, Cell Physiology]

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EID
305549
EOID
806200
Map
0
LastModified
2016年2月1日(月) 10:48:57
Operator
三木 ちひろ
Avail
TRUE
Censor
0
Owner
宮本 理人
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 宮本 理人
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Egawa Tatsuro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Oshima Rieko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Kurogi Eriko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Tomida Yosuke
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Tsuchiya Koichiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Hayashi Tatsuya
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) AICAR stimulation metabolome-widely mimics electrical contraction in isolated rat epitrochlearis muscle.

副題 任意
要約 任意

(英) Physical exercise has potent therapeutic and preventive effects against metabolic disorders. A number of studies have suggested that 5'-AMP-activated protein kinase (AMPK) plays a pivotal role in regulating carbohydrate and lipid metabolism in contracting skeletal muscles, while several genetically manipulated animal models revealed the significance of AMPK-independent pathways. To elucidate significance of AMPK and AMPK-independent signals in contracting skeletal muscles, we conducted a metabolomic analysis that compared the metabolic effects of 5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside (AICAR) stimulation with the electrical contraction ex vivo in isolated rat epitrochlearis muscles, in which both α1- and α2-isoforms of AMPK and glucose uptake were equally activated. The metabolomic analysis using capillary electrophoresis time-of-flight mass spectrometry detected 184 peaks and successfully annotated 132 small molecules. AICAR stimulation exhibited high similarity to the electrical contraction in overall metabolites. Principal component analysis (PCA) demonstrated that the major principal component characterized common effects whereas the minor principal component distinguished the difference. PCA and a factor analysis suggested a substantial change in redox status as a result of AMPK activation. We also found a decrease in reduced glutathione levels in both AICAR-stimulated and contracting muscles. The muscle contraction-evoked influences related to the metabolism of amino acids, in particular, aspartate, alanine, or lysine, are supposed to be independent of AMPK activation. Our results substantiate the significance of AMPK activation in contracting skeletal muscles and provide novel evidence that AICAR stimulation closely mimics the metabolomic changes in the contracting skeletal muscles.

キーワード 推奨
  1. (英) AMP-Activated Protein Kinases
  2. (英) Aminoimidazole Carboxamide
  3. (英) Animals
  4. (英) Cell Line
  5. (英) Electric Stimulation
  6. (英) Factor Analysis, Statistical
  7. グルコース(glucose)
  8. (英) Glutathione
  9. (英) Hypoglycemic Agents
  10. (英) Male
  11. (英) Metabolome
  12. (英) Muscle Contraction
  13. (英) Muscle, Skeletal
  14. (英) Principal Component Analysis
  15. (英) Random Allocation
  16. (英) Rats
  17. (英) Rats, Sprague-Dawley
  18. (英) Ribonucleotides
  19. (英) Transcriptome
発行所 推奨
誌名 必須 American Journal of Physiology, Cell Physiology([アメリカ生理学会])
(pISSN: 0363-6143, eISSN: 1522-1563)
ISSN 任意 1522-1563
ISSN: 0363-6143 (pISSN: 0363-6143, eISSN: 1522-1563)
Title: American journal of physiology. Cell physiology
Title(ISO): Am J Physiol Cell Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 305
必須 12
必須 1214 1222
都市 任意
年月日 必須 2014年 1月 14日
URL 任意
DOI 任意 10.1152/ajpcell.00162.2013    (→Scopusで検索)
PMID 任意 24088893    (→Scopusで検索)
NAID 任意
WOS 任意 000328760800005
Scopus 任意 2-s2.0-84890390626
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1152/ajpcell.00162.2013

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) KeywordList.Keyword: 5'-AMP-activated protein kinase

  5. (英) KeywordList.Keyword: AICAR

  6. (英) KeywordList.Keyword: isolated skeletal muscle

  7. (英) KeywordList.Keyword: metabolome

  8. (英) KeywordList.Keyword: muscle contraction