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著作: [佐藤 克也]/OGAWA Yuki/[伊藤 伸一]/[藤澤 正一郎]/MINAMI Kazuyuki/Strain magnitude dependent intracellular calcium signaling response to uniaxial stretch in osteoblastic cells/[Journal of Biomechanical Science and Engineering]

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EID
305442
EOID
913382
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0
LastModified
2018年7月8日(日) 21:01:07
Operator
大家 隆弘
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Owner
佐藤 克也
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 日本機械学会
著者 必須
  1. 佐藤 克也([徳島大学.大学院社会産業理工学研究部.理工学域.機械科学系.知能機械学分野]/[徳島大学.理工学部.理工学科.機械科学コース.知能機械学講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) OGAWA Yuki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 伊藤 伸一([徳島大学.大学院社会産業理工学研究部.理工学域.知能情報系.知能工学分野]/[徳島大学.理工学部.理工学科.情報光システムコース.知能工学講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 藤澤 正一郎
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) MINAMI Kazuyuki
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Strain magnitude dependent intracellular calcium signaling response to uniaxial stretch in osteoblastic cells

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要約 任意

(英) In osteoblast cells, cells change their intracellular Ca2+ concentration ([Ca2+]i) as the response to mechanical stimuli. Although it has been reported that osteoblast cells responded to many kinds of mechanical stimuli including stretch of substrate, shear stress in fluid flow, direct indentation of glass microneedle and hydrostatic pressure etc., the detail of the characteristics of intracellular calcium signaling response to substrate stretch still remains unclear because motion artifact during stretch application causes out of focus plane and observation area, and complicates the in situ time lapse observation of change in [Ca2+]i in a single cell level. In this study, we combined our originally developed cell stretching MEMS device with the ratiometric microscopy method with two kinds of visible wavelength calcium indicator dyes. The cell stretching micro device and the ratiometric method reduce the influence of motion artifact during stretch application, and enable us to quantitatively evaluate the characteristics of cellular calcium signaling response to stretch. MC3T3-E1 osteoblastic cells were plated onto the cell stretching micro device and fluorescently labeled by Ca2+ indicator Fluo 3 and Fura Red. A uniaxial stretch with three magnitudes of strain 5%, 10% and 15% with constant strain rate were applied to the cells, and in situ time lapse observation of cellular calcium signaling response to stretch was conducted with high temporal and spatial resolution. We succeeded in obtaining time lapse fluorescent image sequences during stretch application without excessive out of focus and blank time. The results revealed that MC3T3-E1 cells change the intensity of calcium signaling response to stretch according to the stretch strain magnitude. As stretch strain magnitude was increased, the amount of change in fluorescent ratio value of Ca2+ indicators in stretched cells also increased. This result suggests the possibility that osteoblastic cells can sense the magnitude of mechanical stimuli at upstream of mechanotransduction pathway such as influx of extracellular Ca2+.

キーワード 推奨
  1. (英) Cell Biomechanics
  2. (英) Mechanotransduction
  3. 骨芽細胞(osteoblast)
  4. 細胞内カルシウム(intracellular calcium)
  5. (英) Stretch stimuli
発行所 推奨 日本機械学会
誌名 必須 Journal of Biomechanical Science and Engineering([日本機械学会])
(eISSN: 1880-9863)
ISSN 任意 1880-9863
ISSN: 1880-9863 (eISSN: 1880-9863)
Title: Journal of Biomechanical Science and Engineering
Supplier: 一般社団法人 日本機械学会
Publisher: Japan Society of Mechanical Engineers/Nihon Kikai Gakkai
 (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 10
必須 3
必須 1 11
都市 任意
年月日 必須 2015年 9月 3日
URL 任意 http://ci.nii.ac.jp/naid/130005097340/
DOI 任意 10.1299/jbse.15-00242    (→Scopusで検索)
PMID 任意
NAID 任意 130005109062
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意