徳島大学 教育・研究者情報データベース(EDB)

1. (英) Ikawa H

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2. 山本 圭([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生命科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])

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3. (英) Takahashi Y

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4. (英) Ueda N

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5. (英) Hayashi Y

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6. (英) Yamamoto S

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7. (英) Ishimura K

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8. (英) Irahara M

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9. (英) Aono T

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(英) Arachidonate 12-lipoxygenase in porcine anterior pituitary cells; its localization and possible function in gonadotrophs.

(英) Arachidonate 12-lipoxygenase, which oxygenates positions 12 and 13 of arachidonic and linoleic acids, is present in porcine anterior pituitary cells. Colocalization of the 12-lipoxygenase with various pituitary hormones was examined by immunohistochemical double-staining using antibodies against 12-lipoxygenase and various anterior pituitary hormones. Under light microscopy, approximately 7% of the cells producing luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were positive for 12-lipoxygenase, whereas the enzyme was detected in less than 2% of the cells producing thyrotrophin, prolactin, growth hormone (GH), and adrenocorticotrophin. In an attempt to examine the participation of 12-lipoxygenase metabolites in pituitary hormone release, we incubated the primary culture of porcine anterior pituitary cells with 12-hydroperoxy-arachidonic acid or 13-hydroperoxy-linoleic acid. Significant stimulation of LH and FSH release by these hydroperoxides was observed at 10 microM in a time-dependent manner. At doses around 10 microM these compounds produced responses of similar magnitude to 1 nM gonadotrophin-releasing hormone (GnRH), but higher concentrations (30 microM) of the compounds were required for GH release. In contrast, 12-hydroxy-arachidonic and 13-hydroxy-linoleic acids were almost ineffective. Furthermore, the gonadotrophin release by 1 nM GnRH was inhibited by nordihydroguaiaretic acid (a lipoxygenase inhibitor) with an IC50 of about 5 microM. Thus, the hydroperoxy (but not hydroxy) products of 12-lipoxygenase may be involved in the release of pituitary hormones especially LH and FSH.

1. (英) 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
2. (英) Animals
3. (英) Antithrombins
4. (英) Arachidonate 12-Lipoxygenase
5. (英) Cells, Cultured
6. (英) Chromatography, High Pressure Liquid
7. (英) Fatty Acids, Unsaturated
8. (英) Follicle Stimulating Hormone
9. (英) Gonadotropin-Releasing Hormone
10. (英) Growth Hormone
11. (英) Hydroxyeicosatetraenoic Acids
12. 免疫組織化学(immunohistochemistry)
13. (英) Leukotrienes
14. (英) Linoleic Acids
15. (英) Lipid Peroxides
16. (英) Luteinizing Hormone
17. (英) Pituitary Gland, Anterior
18. (英) Swine
19. (英) Time Factors

1. (英) Article.PublicationTypeList.PublicationType: Journal Article

2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't