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著作: Oh Yukiko/Oh Iekuni/[森本 純子]/Uede Toshimitsu/Morimoto Akira/Osteopontin has a crucial role in osteoclast-like multinucleated giant cell formation./[Journal of Cellular Biochemistry]

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EID
300666
EOID
797351
Map
0
LastModified
2015年9月10日(木) 11:36:11
Operator
大家 隆弘
Avail
TRUE
Censor
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Owner
森本 純子
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種別 必須
言語 必須 英語
招待 推奨
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共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Oh Yukiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Oh Iekuni
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 森本 純子([徳島大学.先端酵素学研究所.重点研究部門])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Uede Toshimitsu
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Morimoto Akira
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Osteopontin has a crucial role in osteoclast-like multinucleated giant cell formation.

副題 任意
要約 任意

(英) The osteoclast (OC) is a major player in the pathogenic bone destruction of inflammatory bone diseases such as rheumatoid arthritis and Langerhans cell histiocytosis. Recently, it was shown that immature dendritic cells (iDC) fuse faster and more efficiently than monocytes in forming OC-like multinucleated giant cells (MGCs), and that osteopontin (OPN) is involved in the pathogenesis of inflammatory bone diseases. In this study, we hypothesized that OPN is a key factor for generation of OC-like MGCs from iDCs. We used an in vitro culture system to differentiate iDCs, derived from monocytes obtained from the blood of healthy donors, into OC-like MGCs. We evaluated OPN levels and expression of OPN receptors during the course of differentiation. OPN has an arginine-glycine-aspartic acid (RGD) motif, and protease cleavage reveals a SVVYGLR motif. The concentrations of both full-length and cleaved forms of OPN increased during the course of OC-like MGC formation. Expression of OPN RGD- and SVVYGLR-recognizing receptors also increased at later stages. We analyzed whether blocking OPN binding to its receptors affected OC-like MGC formation. Monocytes treated with OPN siRNA were able to differentiate into iDCs effectively; however, differentiation of these iDCs into OC-like MGCs was significantly reduced. The formation of OC-like MGCs was not significantly reduced by RGD synthetic peptide. By contrast, SVVYGLR synthetic peptide caused a significant reduction. These data suggest that the cleaved form of OPN plays a critical role in driving iDC differentiation into OC-like MGCs in the early phase of differentiation, in an autocrine and/or paracrine fashion.

キーワード 推奨
  1. (英) Arthritis, Rheumatoid
  2. (英) Cell Differentiation
  3. (英) Dendritic Cells
  4. (英) Giant Cells
  5. (英) Histiocytosis, Langerhans-Cell
  6. (英) Humans
  7. (英) Oligopeptides
  8. (英) Osteoclasts
  9. (英) Osteopontin
  10. (英) RNA, Small Interfering
発行所 推奨
誌名 必須 Journal of Cellular Biochemistry([Wiley-Liss, Inc.])
(pISSN: 0730-2312, eISSN: 1097-4644)
ISSN 任意 1097-4644
ISSN: 0730-2312 (pISSN: 0730-2312, eISSN: 1097-4644)
Title: Journal of cellular biochemistry
Title(ISO): J Cell Biochem
Publisher: Wiley
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 115
必須 3
必須 585 595
都市 任意
年月日 必須 2014年 3月 初日
URL 任意
DOI 任意 10.1002/jcb.24695    (→Scopusで検索)
PMID 任意 24129963    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1002/jcb.24695

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) KeywordList.Keyword: IMMATURE DENDRITIC CELL

  5. (英) KeywordList.Keyword: INFLAMMATORY BONE DISEASE

  6. (英) KeywordList.Keyword: MULTINUCLEATED GIANT CELL

  7. (英) KeywordList.Keyword: OSTEOCLAST

  8. (英) KeywordList.Keyword: OSTEOPONTIN