徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [岡村 永一]/Matsuzaki Hitomi/Fukamizu Akiyoshi/Tanimoto Keiji/The chicken HS4 insulator element does not protect the H19 ICR from differential DNA methylation in yeast artificial chromosome transgenic mouse./[PLoS ONE]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
300231
EOID
795892
Map
0
LastModified
2015年9月4日(金) 15:12:29
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
岡村 永一
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 岡村 永一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Matsuzaki Hitomi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Fukamizu Akiyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Tanimoto Keiji
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) The chicken HS4 insulator element does not protect the H19 ICR from differential DNA methylation in yeast artificial chromosome transgenic mouse.

副題 任意
要約 任意

(英) Mono-allelic expression at the mouse IGF2/H19 locus is controlled by differential allelic DNA methylation of the imprinting control region (ICR). Because a randomly integrated H19 ICR fragment, when incorporated into the genome of transgenic mice (TgM), was allele-specifically methylated in somatic, but not in germ cells, it was suggested that allele-discriminating epigenetic signature, set within or somewhere outside of the Tg H19 ICR fragment in germ cells, was later translated into a differential DNA methylation pattern. To test if the chicken β-globin HS4 (cHS4) chromatin insulator might interfere with methylation imprinting establishment at the H19 ICR, we inserted the H19 ICR fragment, flanked by a set of floxed cHS4 core sequences, into a human β-globin locus YAC and generated TgM (insulated ICR' TgM). As controls, the cHS4 sequences were removed from one side (5'HS4-deleted ICR') or both sides (pseudo-WT ICR') of the insulated ICR' by in vivo cre-loxP recombination. The data show that while maternally inherited transgenic H19 ICR was not methylated in insulated ICR' TgM, it was significantly methylated upon paternal transmission, though the level was lower than in the pseudo-WT ICR' control. Because this reduced level of methylation was also observed in the 5'HS4-deleted ICR' TgM, we speculate that the phenotype is due to VEZF1-dependent demethylation activity, rather than the insulator function, borne in cHS4. Collectively, although we cannot rule out the possibility that cHS4 is incapable of blocking an allele-discriminating signal from outside of the transgene, the epigenetic signature appears to be marked intrinsically within the H19 ICR.

キーワード 推奨
  1. (英) Animals
  2. (英) Chickens
  3. (英) Chromosomes, Artificial
  4. (英) DNA Methylation
  5. (英) Genomic Imprinting
  6. (英) Humans
  7. (英) Insulator Elements
  8. (英) Insulin-Like Growth Factor II
  9. (英) Mice
  10. (英) Mice, Transgenic
  11. (英) Saccharomyces cerevisiae
発行所 推奨
誌名 必須 PLoS ONE(Public Library of Science)
(eISSN: 1932-6203)
ISSN 任意 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: PLOS
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 8
必須 9
必須 e73925 e73925
都市 任意
年月日 必須 2013年 9月 4日
URL 任意
DOI 任意 10.1371/journal.pone.0073925    (→Scopusで検索)
PMID 任意 24023912    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1371/journal.pone.0073925

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC3762768