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著作: Sakaguchi Ryuuta/[岡村 永一]/Matsuzaki Hitomi/Fukamizu Akiyoshi/Tanimoto Keiji/Sox-Oct motifs contribute to maintenance of the unmethylated H19 ICR in YAC transgenic mice./[Human Molecular Genetics]

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EID
300230
EOID
795889
Map
0
LastModified
2015年9月4日(金) 15:10:04
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
岡村 永一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Sakaguchi Ryuuta
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 岡村 永一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Matsuzaki Hitomi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Fukamizu Akiyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Tanimoto Keiji
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Sox-Oct motifs contribute to maintenance of the unmethylated H19 ICR in YAC transgenic mice.

副題 任意
要約 任意

(英) Abnormal methylation at the maternally inherited H19 imprinted control region (H19 ICR) is one of the causative alterations leading to pathogenesis of Beckwith-Wiedemann syndrome (BWS). Recently, it was shown in human BWS patients, as well as mouse cell culture experiments, that Sox-Oct motifs (SOM) in the H19 ICR might play a role in protecting the maternal ICR from de novo DNA methylation. By grafting a mouse H19 ICR fragment into a human β-globin yeast artificial chromosome (YAC) followed by analysis in transgenic mice (TgM), we showed previously that the fragment carried sufficient information to establish and maintain differential methylation after fertilization. To examine possible functions of the SOM in the establishment and/or maintenance of differential methylation, two kinds of YAC-TgM were generated in this study. In the ΔSOM TgM, carrying the mouse H19 ICR bearing an SOM deletion, a maternally inherited transgenic ICR exhibited increased levels of methylation around the deletion site, in comparison to the wild-type control, after implantation. In the λ + CTCF + b (LCb) TgM, carrying a 2.3 kb λ DNA fragment supplemented with the fragment b including the SOM and four CTCF binding sites, maternally and some of the paternally inherited LCb fragments were significantly less methylated when compared with a control λ + CTCF fragment that was supplemented only with additional CTCF sites; the λ + CTCF was substantially methylated regardless of the parent of origin after implantation. These results demonstrated that the SOM in the maternal H19 ICR was required for maintaining surrounding sequences in the unmethylated state in vivo.

キーワード 推奨
  1. (英) Alleles
  2. (英) Amino Acid Motifs
  3. (英) Animals
  4. (英) Beckwith-Wiedemann Syndrome
  5. (英) Chromosomes, Artificial, Yeast
  6. (英) DNA Methylation
  7. (英) Female
  8. (英) Genomic Imprinting
  9. (英) Humans
  10. (英) Insulin-Like Growth Factor II
  11. (英) Locus Control Region
  12. (英) Male
  13. (英) Mice
  14. (英) Mice, Inbred ICR
  15. (英) Mice, Transgenic
  16. (英) Pedigree
  17. (英) Repressor Proteins
  18. (英) beta-Globins
発行所 推奨
誌名 必須 Human Molecular Genetics([Oxford University Press])
(pISSN: 0964-6906, eISSN: 1460-2083)
ISSN 任意 1460-2083
ISSN: 0964-6906 (pISSN: 0964-6906, eISSN: 1460-2083)
Title: Human molecular genetics
Title(ISO): Hum Mol Genet
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 22
必須 22
必須 4627 4637
都市 任意
年月日 必須 2013年 7月 2日
URL 任意
DOI 任意 10.1093/hmg/ddt311    (→Scopusで検索)
PMID 任意 23821645    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1093/hmg/ddt311

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't