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著作: [岡村 永一]/Matsuzaki Hitomi/Sakaguchi Ryuuta/Takahashi Takuya/Fukamizu Akiyoshi/Tanimoto Keiji/The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice./[Molecular and Cellular Biology]

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EID
300229
EOID
797507
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0
LastModified
2015年9月10日(木) 17:06:48
Operator
大家 隆弘
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Owner
岡村 永一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 岡村 永一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Matsuzaki Hitomi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Sakaguchi Ryuuta
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Takahashi Takuya
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Fukamizu Akiyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Tanimoto Keiji
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice.

副題 任意
要約 任意

(英) In the mouse Igf2/H19 imprinted locus, differential methylation of the imprinting control region (H19 ICR) is established during spermatogenesis and is maintained in offspring throughout development. Previously, however, we observed that the paternal H19 ICR, when analyzed in yeast artificial chromosome transgenic mice (YAC-TgM), was preferentially methylated only after fertilization. To identify the DNA sequences that confer methylation imprinting, we divided the H19 ICR into two fragments (1.7 and 1.2 kb), ligated them to both ends of a λ DNA fragment into which CTCF binding sites had been inserted, and analyzed this in YAC-TgM. The maternally inherited λ sequence, normally methylated after implantation in the absence of H19 ICR sequences, became hypomethylated, demonstrating protective activity against methylation within the ICR. Meanwhile, the paternally inherited λ sequence was hypermethylated before implantation only when a 1.7-kb fragment was ligated. Consistently, when two subfragments of the H19 ICR were individually investigated for their activities in YAC-TgM, only the 1.7-kb fragment was capable of introducing paternal allele-specific DNA methylation. These results show that postfertilization methylation imprinting is conferred by a paternal allele-specific methylation activity present in a 1.7-kb DNA fragment of the H19 ICR, while maternal allele-specific activities protect the allele from de novo DNA methylation.

キーワード 推奨
  1. (英) Animals
  2. (英) Base Sequence
  3. (英) Blastocyst
  4. (英) Chromosomes, Artificial, Yeast
  5. (英) DNA Methylation
  6. (英) Female
  7. (英) Fertilization
  8. (英) Genomic Imprinting
  9. (英) Male
  10. (英) Mice
  11. (英) Mice, Transgenic
  12. (英) Proteins
発行所 推奨
誌名 必須 Molecular and Cellular Biology([アメリカ微生物学会])
(pISSN: 0270-7306, eISSN: 1098-5549)
ISSN 任意 1098-5549
ISSN: 0270-7306 (pISSN: 0270-7306, eISSN: 1098-5549)
Title: Molecular and cellular biology
Title(ISO): Mol Cell Biol
Publisher: Taylor & Francis
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 33
必須 4
必須 858 871
都市 任意
年月日 必須 2012年 12月 10日
URL 任意
DOI 任意 10.1128/MCB.01003-12    (→Scopusで検索)
PMID 任意 23230275    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1128/MCB.01003-12

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC3571351