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著作: Matsuzaki Hitomi/[岡村 永一]/Fukamizu Akiyoshi/Tanimoto Keiji/CTCF binding is not the epigenetic mark that establishes post-fertilization methylation imprinting in the transgenic H19 ICR./[Human Molecular Genetics]

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EID
300226
EOID
797823
Map
0
LastModified
2015年9月12日(土) 18:32:59
Operator
大家 隆弘
Avail
TRUE
Censor
0
Owner
岡村 永一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Matsuzaki Hitomi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 岡村 永一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Fukamizu Akiyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Tanimoto Keiji
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) CTCF binding is not the epigenetic mark that establishes post-fertilization methylation imprinting in the transgenic H19 ICR.

副題 任意
要約 任意

(英) Imprinted expression of the mouse Igf2/H19 locus is controlled by parent-of-origin-specific methylation of the imprinting control region (ICR). We previously demonstrated that when placed in a heterologous genomic context, the H19 ICR fragment contains an intrinsic activity that allows it to acquire differential methylation in somatic cells but not in germ cells. In the present study, we investigated the requirements for the CTCF-binding sites of the ICR in the acquisition of post-fertilization methylation. To this end, two mutant ICR fragments were introduced into the human beta-globin locus in a yeast artificial chromosome transgenic mouse (TgM) model: 4xMut had mutations in all four ICR CTCF-binding sites that prevented CTCF binding but retained the methylation target CpG motifs, and -9CG harbored mutations in the CpG motifs within the CTCF-binding sites but each site retained constitutive CTCF-binding activity. In TgM germ cells and pre-implantation blastocysts, the absence of CTCF-binding sites (4xMut) did not lead to hypermethylation of the transgenic H19 ICR. However, after implantation, the mutations of CTCF sites (4xMut and -9CG) affected the maintenance of methylation. These results demonstrated that although the CTCF-binding sites are indispensable for maintenance of the unmethylated state of the maternal ICR in post-implantation embryos, they are not required to establish paternal-allele-specific methylation of the transgenic H19 ICR in pre-implantation embryos.

キーワード 推奨
  1. (英) Animals
  2. (英) Binding Sites
  3. (英) Blastocyst
  4. (英) CpG Islands
  5. (英) DNA Methylation
  6. (英) Embryo, Mammalian
  7. (英) Female
  8. (英) Genomic Imprinting
  9. (英) Humans
  10. (英) Insulin-Like Growth Factor II
  11. (英) Locus Control Region
  12. (英) Male
  13. (英) Mice
  14. (英) Mice, Transgenic
  15. (英) Mutation
  16. (英) Repressor Proteins
  17. (英) Transgenes
  18. (英) beta-Globins
発行所 推奨
誌名 必須 Human Molecular Genetics([Oxford University Press])
(pISSN: 0964-6906, eISSN: 1460-2083)
ISSN 任意 1460-2083
ISSN: 0964-6906 (pISSN: 0964-6906, eISSN: 1460-2083)
Title: Human molecular genetics
Title(ISO): Hum Mol Genet
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 19
必須 7
必須 1190 1198
都市 任意
年月日 必須 2010年 1月 4日
URL 任意
DOI 任意 10.1093/hmg/ddp589    (→Scopusで検索)
PMID 任意 20047949    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1093/hmg/ddp589

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't