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著作: Matsuzaki Hitomi/[岡村 永一]/Shimotsuma Motoshi/Fukamizu Akiyoshi/Tanimoto Keiji/A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells./[Molecular and Cellular Biology]

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EID
300224
EOID
797860
Map
0
LastModified
2015年9月12日(土) 19:35:36
Operator
大家 隆弘
Avail
TRUE
Censor
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Owner
岡村 永一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Matsuzaki Hitomi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 岡村 永一
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Shimotsuma Motoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Fukamizu Akiyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Tanimoto Keiji
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) A randomly integrated transgenic H19 imprinting control region acquires methylation imprinting independently of its establishment in germ cells.

副題 任意
要約 任意

(英) The imprinted expression of the mouse Igf2/H19 locus is governed by the differential methylation of the imprinting control region (ICR), which is established initially in germ cells and subsequently maintained in somatic cells, depending on its parental origin. By grafting a 2.9-kbp H19 ICR fragment into a human beta-globin yeast artificial chromosome in transgenic mice, we previously showed that the ICR could recapitulate imprinted methylation and expression at a heterologous locus, suggesting that the H19 ICR in the beta-globin locus contained sufficient information to maintain the methylation mark (K. Tanimoto, M. Shimotsuma, H. Matsuzaki, A. Omori, J. Bungert, J. D. Engel, and A. Fukamizu, Proc. Natl. Acad. Sci. USA 102:10250-10255, 2005). Curiously, however, the transgenic H19 ICR was not methylated in sperm, which was distinct from that seen in the endogenous locus. Here, we reevaluated the ability of the H19 ICR to mark the parental origin using more rigid criteria. In the testis, the methylation levels of the solitary 2.9-kbp transgenic ICR fragment varied significantly between six transgenic mouse lines. However, in somatic cells, the paternally inherited ICR fragment exhibited consistently higher methylation levels at five out of six randomly integrated sites in the mouse genome. These results clearly demonstrated that the H19 ICR could acquire parent-of-origin-dependent methylation after fertilization independently of the chromosomal integration site or the prerequisite methylation acquisition in male germ cells.

キーワード 推奨
  1. (英) Animals
  2. (英) Cells, Cultured
  3. (英) DNA Methylation
  4. (英) Female
  5. (英) Genomic Imprinting
  6. (英) Germ Cells
  7. (英) Humans
  8. (英) Insulin-Like Growth Factor II
  9. (英) Locus Control Region
  10. (英) Male
  11. (英) Mice
  12. (英) Mice, Transgenic
  13. (英) Pedigree
  14. (英) RNA, Long Noncoding
  15. (英) RNA, Untranslated
  16. (英) Testis
  17. (英) Transgenes
発行所 推奨
誌名 必須 Molecular and Cellular Biology([アメリカ微生物学会])
(pISSN: 0270-7306, eISSN: 1098-5549)
ISSN 任意 1098-5549
ISSN: 0270-7306 (pISSN: 0270-7306, eISSN: 1098-5549)
Title: Molecular and cellular biology
Title(ISO): Mol Cell Biol
Publisher: Taylor & Francis
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 29
必須 17
必須 4595 4603
都市 任意
年月日 必須 2009年 6月 22日
URL 任意
DOI 任意 10.1128/MCB.00275-09    (→Scopusで検索)
PMID 任意 19546235    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1128/MCB.00275-09

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC2725707