徳島大学 教育・研究者情報データベース(EDB)

1. (英) Hotta Fumika

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2. 江口 洋

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3. 内藤 毅

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4. 三田村 佳典([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.眼科学])

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5. (英) Kusujima Kohei

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6. (英) Kuwahara Tomomi

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(英) Achromobacter buckle infection diagnosed by a 16S rDNA clone library analysis: a case report.

(英) In clinical settings, bacterial infections are usually diagnosed by isolation of colonies after laboratory cultivation followed by species identification with biochemical tests. However, biochemical tests result in misidentification due to similar phenotypes of closely related species. In such cases, 16S rDNA sequence analysis is useful. Herein, we report the first case of an Achromobacter-associated buckle infection that was diagnosed by 16S rDNA sequence analysis. This report highlights the significance of Achromobacter spp. in device-related ophthalmic infections. A 56-year-old woman, who had received buckling surgery using a silicone solid tire for retinal detachment eighteen years prior to this study, presented purulent eye discharge and conjunctival hyperemia in her right eye. Buckle infection was suspected and the buckle material was removed. Isolates from cultures of preoperative discharge and from deposits on the operatively removed buckle material were initially identified as Alcaligenes and Corynebacterium species. However, sequence analysis of a 16S rDNA clone library using the DNA extracted from the deposits on the buckle material demonstrated that all of the 16S rDNA sequences most closely matched those of Achromobacter spp. We concluded that the initial misdiagnosis of this case as an Alcaligenes buckle infection was due to the unreliability of the biochemical test in discriminating Achromobacter and Alcaligenes species due to their close taxonomic positions and similar phenotypes. Corynebacterium species were found to be contaminants from the ocular surface. Achromobacter spp. should be recognized as causative agents for device-related ophthalmic infections. Molecular species identification by 16S rDNA sequence analysis should be combined with conventional cultivation techniques to investigate the significance of Achromobacter spp. in ophthalmic infections.

1. (英) Achromobacter
2. (英) Anti-Bacterial Agents
3. (英) Betamethasone
4. (英) Cephalosporins
5. (英) DNA, Bacterial
6. (英) DNA, Ribosomal
7. (英) Device Removal
8. (英) Drug Therapy, Combination
9. (英) Eye Infections, Bacterial
10. (英) Female
11. (英) Fluoroquinolones
12. (英) Glucocorticoids
13. (英) Gram-Negative Bacterial Infections
14. (英) Humans
15. (英) Middle Aged
16. (英) Polymerase Chain Reaction
17. (英) Prosthesis-Related Infections
18. (英) RNA, Ribosomal, 16S
19. (英) Retinal Detachment
20. (英) Scleral Buckling

1. (英) Article.ELocationID: 10.1186/1471-2415-14-142

2. (英) Article.PublicationTypeList.PublicationType: Case Reports

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) OtherID: PMC4255664