徳島大学 教育・研究者情報データベース(EDB)

1. (英) Stacy R. Mitchel

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2. (英) Naito Yuji

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3. (英) Maxfield W. Mark

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4. 黒部 裕嗣

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5. (英) Tara Shuhei

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6. (英) Chan Chung

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7. (英) Rocco A. Kevin

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8. (英) Shinoka Toshiharu

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9. (英) Sinusas J. Albert

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10. (英) Breuer K. Christopher

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(英) Targeted imaging of matrix metalloproteinase activity in the evaluation of remodeling tissue-engineered vascular grafts implanted in a growing lamb model.

(英) The clinical translation of tissue-engineered vascular grafts has been demonstrated in children. The remodeling of biodegradable, cell-seeded scaffolds to functional neovessels has been partially attributed to matrix metalloproteinases. Noninvasive assessment of matrix metalloproteinase activity can indicate graft remodeling and elucidate the progression of neovessel formation. Therefore, matrix metalloproteinase activity was evaluated in grafts implanted in lambs using in vivo and ex vivo hybrid imaging. Graft growth and remodeling was quantified using in vivo x-ray computed tomography angiography. Cell-seeded and unseeded scaffolds were implanted in 5 lambs as inferior vena cava interposition grafts. At 2 and 6 months after implantation, in vivo angiography was used to assess graft morphology. In vivo and ex vivo single photon emission tomography/computed tomography imaging was performed with a radiolabeled compound targeting matrix metalloproteinase activity at 6 months. The neotissue was examined at 6 months using qualitative histologic and immunohistochemical staining and quantitative biochemical analysis. The seeded grafts demonstrated significant luminal and longitudinal growth from 2 to 6 months. In vivo imaging revealed subjectively greater matrix metalloproteinase activity in grafts versus native tissue. Ex vivo imaging confirmed a quantitative increase in matrix metalloproteinase activity and demonstrated greater activity in unseeded versus seeded grafts. The glycosaminoglycan content was increased in seeded grafts versus unseeded grafts, without significant differences in collagen content. Matrix metalloproteinase activity remained elevated in tissue-engineered grafts 6 months after implantation and could indicate remodeling. Optimization of in vivo imaging to noninvasively evaluate matrix metalloproteinase activity could assist in the serial assessment of vascular graft remodeling.

1. (英) Age Factors
2. (英) Animals
3. (英) Bioprosthesis
4. (英) Blood Vessel Prosthesis
5. (英) Blood Vessel Prosthesis Implantation
6. (英) Bone Marrow Cells
7. (英) Bone Marrow Transplantation
8. (英) Cell Proliferation
9. (英) Cells, Cultured
10. コラーゲン(collagen)
11. (英) Feasibility Studies
12. (英) Glycosaminoglycans
13. (英) Matrix Metalloproteinases
14. (英) Models, Animal
15. (英) Multidetector Computed Tomography
16. (英) Phlebography
17. (英) Prosthesis Design
18. (英) Sheep
19. (英) Time Factors
20. 再生医学(tissue engineering)
21. (英) Tissue Scaffolds
22. (英) Tomography, Emission-Computed, Single-Photon
23. (英) Up-Regulation
24. (英) Vascular Remodeling
25. (英) Vena Cava, Inferior

1. (英) Article.ELocationID: 10.1016/j.jtcvs.2014.05.037

2. (英) Article.ELocationID: S0022-5223(14)00581-9

3. (英) Article.PublicationTypeList.PublicationType: Evaluation Studies

4. (英) Article.PublicationTypeList.PublicationType: Journal Article

5. (英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural

6. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't