徳島大学 教育・研究者情報データベース(EDB)

1. (英) Hemdan Dalia Ismaeil Ibrahim

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2. 平坂 勝也

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3. (英) Nakao Reiko

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4. (英) Kohno Shohei

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5. (英) Kagawa Sachiko

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6. 安倍 知紀

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7. 原田 晃子

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8. 奥村 裕司

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9. 中屋 豊

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10. 寺尾 純二

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11. 二川 健([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.生体栄養学])

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(英) Polyphenols prevent clinorotation-induced expression of atrogenes in mouse C2C12 skeletal myotubes.

(英) Oxidative stress is a key factor in stimulating the expression of atrogenes, which are muscle atrophy-related ubiquitin ligases, in skeletal muscle, and it induces muscle atrophy during unloading. However, the effects of antioxidative nutrients on atrogene expression have not been demonstrated. We report on the inhibitory effects of polyphenols, such as epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) and quercetin, on atrogene expression up-regulated by three dimensional (3D)-clinorotation or glucocorticoid. These treatments markedly elevated the expression of atrogenes, including atrogin-1 and MuRF-1, in mouse C2C12 myoblasts and myotubes. Interestingly, EC, ECg, EGCg and quercetin significantly decreased the expression of atrogin-1 and MuRF-1 up-regulated by 3D-clinorotation, whereas they hardly affected atrogene expression induced by dexamethasone. ERK signaling is a well known MAPK pathway to mediate oxidative stress. Therefore, we also investigated the effect of these polyphenols on phosphorylation of ERK in C2C12 myotubes. As expected, EC, ECg, EGCg, and quercetin significantly suppressed phosphorylation of ERK, corresponding to the up-regulation of atrogenes induced by 3D-clinorotation. These results suggest that antioxidative nutrients, such as catechins and quercetin, suppress atrogene expression in skeletal muscle cells, possibly through the inhibition of ERK signaling. Thus, catechins and quercetin may prevent unloading-mediated muscle atrophy.

1. (英) Animals
2. (英) Antioxidants
3. カテキン(catechin)
4. (英) Cell Line
5. (英) Dexamethasone
6. (英) Extracellular Signal-Regulated MAP Kinases
7. フラボノイド(flavonoids)
8. (英) Glucocorticoids
9. (英) Mice
10. (英) Muscle Fibers, Skeletal
11. (英) Muscle Proteins
12. (英) Muscle, Skeletal
13. 酸化ストレス(oxidative stress)
14. (英) Phenols
15. 酸化的リン酸化(oxidative phosphorylation)
16. (英) Quercetin
17. (英) Rotation
18. (英) SKP Cullin F-Box Protein Ligases
19. (英) Ubiquitin-Protein Ligases

1. (英) Affiliation: Department of Nutritional Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School, Japan.

2. (英) PublicationType: Journal Article

3. (英) PublicationType: Research Support, Non-U.S. Gov't