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著作: [川上 竜巳]/[櫻庭 春彦]/[大島 敏久]/Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis/[Bioscience, Biotechnology, and Biochemistry]

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EID
290230
EOID
796974
Map
0
LastModified
2015年9月8日(火) 20:40:51
Operator
大家 隆弘
Avail
TRUE
Censor
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Owner
川上 竜巳
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨 研究
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 川上 竜巳([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 櫻庭 春彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 大島 敏久([九州大学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis

副題 任意
要約 任意

(英) We previously found a very large NAD-dependent glutamate dehydrogenase with approximately 170 kDa subunit from Janthinobacterium lividum (Jl-GDH) and predicted that GDH reaction occurred in the central domain of the subunit. To gain further insights into the role of the central domain, several single point mutations were introduced. The enzyme activity was completely lost in all single mutants of R784A, K810A, K820A, D885A, and S1142A. Because, in sequence alignment analysis, these residues corresponded to the residues responsible for glutamate binding in well-known small GDH with approximately 50 kDa subunit, very large GDH and well-known small GDH may share the same catalytic mechanism. In addition, we demonstrated that C1141, one of the three cysteine residues in the central domain, was responsible for the inhibition of enzyme activity by HgCl2, and HgCl2 functioned as an activating compound for a C1141T mutant. At low concentrations, moreover, HgCl2 was found to function as an activating compound for a wild-type Jl-GDH. This suggests that the mechanism for the activation is entirely different from that for the inhibition.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Bacterial Proteins
  3. (英) Binding Sites
  4. (英) Biocatalysis
  5. (英) Burkholderiaceae
  6. (英) Cysteine
  7. (英) Enzyme Assays
  8. (英) Gene Expression
  9. (英) Glutamate Dehydrogenase
  10. (英) Glutamic Acid
  11. (英) Kinetics
  12. (英) Mercuric Chloride
  13. (英) Molecular Sequence Data
  14. (英) Mutagenesis, Site-Directed
  15. (英) Mutation
  16. (英) Protein Binding
  17. (英) Protein Structure, Tertiary
  18. (英) Protein Subunits
  19. (英) Sequence Homology, Amino Acid
  20. (英) Structure-Activity Relationship
発行所 推奨
誌名 必須 Bioscience, Biotechnology, and Biochemistry([社団法人 日本農芸化学会])
(pISSN: 0916-8451, eISSN: 1347-6947)
ISSN 任意 1347-6947
ISSN: 0916-8451 (pISSN: 0916-8451, eISSN: 1347-6947)
Title: Bioscience, biotechnology, and biochemistry
Title(ISO): Biosci Biotechnol Biochem
Supplier: 社団法人日本農芸化学会
Publisher: Taylor & Francis
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 78
必須 12
必須 2045 2050
都市 任意
年月日 必須 2014年 12月 初日
URL 任意
DOI 任意 10.1080/09168451.2014.946394    (→Scopusで検索)
PMID 任意 25126984    (→Scopusで検索)
NAID 任意
WOS 任意 000345504400010
Scopus 任意 2-s2.0-84924529811
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1080/09168451.2014.946394

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) KeywordList.Keyword: Janthinobacterium lividum

  4. (英) KeywordList.Keyword: activation by Hg2+

  5. (英) KeywordList.Keyword: glutamate-binding residues

  6. (英) KeywordList.Keyword: inhibition by Hg2+

  7. (英) KeywordList.Keyword: very large glutamate dehydrogenase