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著作: Yamazoe Mitsuyoshi/[足立 隼]/Kanaya Shigehiko/Ohsumi Katsufumi/Hiraga Sota/Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli./[Molecular Microbiology]

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EID
290089
EOID
849662
Map
0
LastModified
2017年3月8日(水) 11:58:49
Operator
三木 ちひろ
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Owner
[研究部長]/[徳島大学.大学院医歯薬学研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Yamazoe Mitsuyoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 足立 隼
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Kanaya Shigehiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Ohsumi Katsufumi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Hiraga Sota
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Sequential binding of SeqA protein to nascent DNA segments at replication forks in synchronized cultures of Escherichia coli.

副題 任意
要約 任意

(英) To demonstrate that sequestration A (SeqA) protein binds preferentially to hemimethylated GATC sequences at replication forks and forms clusters in Escherichia coli growing cells, we analysed, by the chromatin immunoprecipitation (ChIP) assay using anti-SeqA antibody, a synchronized culture of a temperature-sensitive dnaC mutant strain in which only one round of chromosomal DNA replication was synchronously initiated. After synchronized initiation of chromosome replication, the replication origin oriC was first detected by the ChIP assay, and other six chromosomal regions having multiple GATC sequences were sequentially detected according to bidirectional replication of the chromosome. In contrast, DNA regions lacking the GATC sequence were not detected by the ChIP assay. These results indicate that SeqA binds hemimethylated nascent DNA segments according to the proceeding of replication forks in the chromosome, and SeqA releases from the DNA segments when fully methylated. Immunofluorescence microscopy reveals that a single SeqA focus containing paired replication apparatuses appears at the middle of the cell immediately after initiation of chromosome replication and the focus is subsequently separated into two foci that migrate to 1/4 and 3/4 cellular positions, when replication forks proceed bidirectionally an approximately one-fourth distance from the replication origin towards the terminus. This supports the translocating replication apparatuses model.

キーワード 推奨
  1. (英) Bacterial Outer Membrane Proteins
  2. (英) Chromatin Immunoprecipitation
  3. (英) DNA Replication
  4. (英) DNA, Bacterial
  5. (英) DNA-Binding Proteins
  6. 大腸菌(Escherichia coli)
  7. (英) Escherichia coli Proteins
  8. (英) Kinetics
  9. (英) Microscopy, Fluorescence
  10. (英) Protein Binding
  11. (英) Staining and Labeling
発行所 推奨
誌名 必須 Molecular Microbiology([Blackwell Publishing Ltd])
(pISSN: 0950-382X, eISSN: 1365-2958)
ISSN 任意 0950-382X
ISSN: 0950-382X (pISSN: 0950-382X, eISSN: 1365-2958)
Title: Molecular microbiology
Title(ISO): Mol Microbiol
Publisher: Blackwell
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 55
必須 1
必須 289 298
都市 任意
年月日 必須 2005年 1月 初日
URL 任意
DOI 任意 10.1111/j.1365-2958.2004.04389.x    (→Scopusで検索)
PMID 任意 15612935    (→Scopusで検索)
NAID 任意
WOS 任意 000225949300025
Scopus 任意 2-s2.0-12344308528
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article

  2. (英) PublicationType: Research Support, Non-U.S. Gov't