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著作: [足立 隼]/Fukushima Toru/Hiraga Sota/Dynamic events of sister chromosomes in the cell cycle of Escherichia coli./[Genes to Cells]

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EID
290086
EOID
849690
Map
0
LastModified
2017年3月8日(水) 14:23:34
Operator
三木 ちひろ
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Owner
[研究部長]/[徳島大学.大学院医歯薬学研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 足立 隼
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Fukushima Toru
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Hiraga Sota
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Dynamic events of sister chromosomes in the cell cycle of Escherichia coli.

副題 任意
要約 任意

(英) Various events involving partitioning of sister chromosomes were precisely analyzed in asynchronously growing Escherichia coli cells in various conditions. To examine the cohesion between sister chromosomes, we analyzed living cells growing under various conditions for the number of the replication origin (oriC) copies by flow cytometry and the foci of oriC by fluorescence microscopy. The average number of the oriC foci per cell was significantly smaller than the number of oriC copies per cell with few exceptions, suggesting cohesion of oriC sister copies. Cohesion phenomenon of oriC sister copies was also observed in a mukB null mutant cells under some growth conditions. Sister copies of the terminal region (ter) were also found to be cohesive. Immunofluorescence microscopy for nascent DNA pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU) indicated that paired replication forks acting on bidirectional replication were able to migrate toward opposite directions during ongoing replication in poor medium; however, some of them were closely associated in rich media. Analysis of the foci of MukB-GFP indicates that the number of MukB foci was always larger than the number of replication forks. The number of MukB-GFP foci increased together with cell length. The sequence of these chromosomal events in the growing cells has been depicted.

キーワード 推奨
  1. (英) Bacterial Outer Membrane Proteins
  2. (英) Bromodeoxyuridine
  3. 細胞周期(cell cycle)
  4. (英) Chromosomal Proteins, Non-Histone
  5. (英) Chromosomes, Bacterial
  6. (英) DNA, Bacterial
  7. (英) DNA-Binding Proteins
  8. 大腸菌(Escherichia coli)
  9. (英) Escherichia coli Proteins
  10. (英) Flow Cytometry
  11. (英) Gene Dosage
  12. (英) Genes, Bacterial
  13. (英) Green Fluorescent Proteins
  14. (英) In Situ Hybridization, Fluorescence
  15. (英) Microscopy, Fluorescence
  16. (英) Mutation
  17. (英) Origin Recognition Complex
  18. (英) Recombinant Fusion Proteins
発行所 推奨
誌名 必須 Genes to Cells([日本分子生物学会])
(pISSN: 1356-9597, eISSN: 1365-2443)
ISSN 任意 1365-2443
ISSN: 1356-9597 (pISSN: 1356-9597, eISSN: 1365-2443)
Title: Genes to cells : devoted to molecular & cellular mechanisms
Title(ISO): Genes Cells
Supplier: Molecular Biology Society of Japan
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 13
必須 2
必須 181 197
都市 任意
年月日 必須 2008年 2月 初日
URL 任意
DOI 任意 10.1111/j.1365-2443.2007.01157.x    (→Scopusで検索)
PMID 任意 18233960    (→Scopusで検索)
NAID 任意
WOS 任意 000252774800007
Scopus 任意 2-s2.0-38849135690
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article

  2. (英) PublicationType: Research Support, Non-U.S. Gov't