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著作: Ashida Y/Ueno A/Miwa Y/[三好 圭子]/Inoue H/Putrescine-stimulated intracellular Ca2+ release for invasiveness of rat ascites hepatoma cells./[Gann : Japanese Journal of Cancer Research]

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EID
286332
EOID
749816
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0
LastModified
2014年10月6日(月) 13:10:52
Operator
三好 圭子
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三好 圭子
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
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著者 必須
  1. (英) Ashida Y
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Ueno A
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Miwa Y
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  4. 三好 圭子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Inoue H
    役割 任意
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題名 必須

(英) Putrescine-stimulated intracellular Ca2+ release for invasiveness of rat ascites hepatoma cells.

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(英) Our previous study showed that treatment of highly invasive rat ascites hepatoma (LC-AH) cells with alpha-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase, decreased both their intracellular level of putrescine and their in vitro invasion of a monolayer of calf pulmonary arterial endothelial (CPAE) cells, and that both these decreases were completely reversed by exogenous putrescine, but not spermidine or spermine. Here we show that all adhering control (DFMO-untreated) cells migrated beneath CPAE monolayer with morphological change from round to cauliflower-shaped cells (migratory cells). DFMO treatment increased the number of cells that remained round without migration (nonmigratory cells). Exogenous putrescine, but not spermidine or spermine, induced transformation of all nonmigratory cells to migratory cells with a concomitant increase in their intracellular Ca2+ level, [Ca2+]i. The putrescine-induced increase in their [Ca2+]i preceded their transformation and these effects of putrescine were not affected by antagonists of the voltage-gated Ca2+ channel, but were completely suppressed by ryanodine, which also suppressed the invasiveness of the control cells. The DFMO-induced decreases in both [Ca2+]i and the invasiveness of the cells were restored by thapsigargin, which elevated [Ca2+]i by inhibiting endoplasmic Ca2+-ATPase, indicating that thapsigargin mimics the effects of putrescine. These results support the idea that putrescine is a cofactor for Ca2+ release through the Ca2+ channel in the endoplasmic reticulum that is inhibited by ryanodine, this release being initiated by cell adhesion and being a prerequisite for tumor cell invasion.

キーワード 推奨
  1. (英) Animals
  2. (英) Ascites
  3. (英) Calcium
  4. (英) Calcium Channel Agonists
  5. (英) Calcium Channel Blockers
  6. (英) Carcinoma, Hepatocellular
  7. (英) Cell Adhesion
  8. (英) Cell Movement
  9. (英) Eflornithine
  10. (英) Enzyme Inhibitors
  11. (英) Liver Neoplasms, Experimental
  12. (英) Membrane Fluidity
  13. (英) Microscopy, Electron, Scanning
  14. (英) Neoplasm Invasiveness
  15. (英) Putrescine
  16. (英) Rats
  17. (英) Spermidine
  18. (英) Spermine
  19. (英) Tumor Cells, Cultured
発行所 推奨
誌名 必須 Gann : Japanese Journal of Cancer Research([日本癌学会])
(pISSN: 0910-5050)
ISSN 任意 0910-5050
ISSN: 0910-5050 (pISSN: 0910-5050, eISSN: 1876-4673)
Title: Japanese journal of cancer research : Gann
Title(ISO): Jpn J Cancer Res
Supplier: 日本癌学会
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 89
必須 1
必須 67 75
都市 任意
年月日 必須 1998年 1月 初日
URL 任意
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PMID 任意 9510478    (→Scopusで検索)
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  1. (英) PublicationType: Journal Article