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著作: Walton K D/Wagner K U/Rucker E B/Shillingford J M/[三好 圭子]/Hennighausen L/Conditional deletion of the bcl-x gene from mouse mammary epithelium results in accelerated apoptosis during involution but does not compromise cell function during lactation./[Mechanisms of Development]

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EID
286330
EOID
749812
Map
0
LastModified
2014年10月6日(月) 13:06:11
Operator
三好 圭子
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TRUE
Censor
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Owner
三好 圭子
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Walton K D
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Wagner K U
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Rucker E B
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Shillingford J M
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 三好 圭子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Hennighausen L
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Conditional deletion of the bcl-x gene from mouse mammary epithelium results in accelerated apoptosis during involution but does not compromise cell function during lactation.

副題 任意
要約 任意

(英) In the mammary gland Bcl-x is the most abundant cell survival factor from the Bcl-2 family. Since Bcl-x null mice die around day 12 of embryogenesis, the relevance of this protein in organ development and function is poorly understood. In erythroid cells bcl-x gene expression is controlled by cytokines and the transcription factor Stat5 (signal transducer and activator of transcription). However, we identified that bcl-x RNA levels in mammary tissue from prolactin receptor- and Stat5-null mice were indistinguishable from wild type mice. We have proposed that Bcl-x might control the survival of mammary epithelial cells throughout pregnancy, lactation, and the early stages of involution, and we have now tested this hypothesis through the conditional deletion of the bcl-x gene from mouse mammary epithelium. Conditional (floxed) bcl-x alleles were excised from alveolar cells during pregnancy using a Cre transgene under the control of the whey acidic protein gene promoter. Deletion of the bcl-x gene from the entire epithelial compartment (ducts and alveoli) was achieved by expressing Cre-recombinase under control of the mouse mammary tumor virus long terminal repeat. The absence of Bcl-x did not compromise proliferation and differentiation of mammary ductal and alveolar epithelial cells in virgin mice and during pregnancy and lactation. However, epithelial cell death and tissue remodeling were accelerated in the bcl-x conditional knockout mice during the first stage of involution. Concomitant deletion of the bax gene did not significantly modify the Bcl-x phenotype. Our results suggest that Bcl-x is not essential during mammopoiesis, but is critical for controlled apoptosis during the first phase of involution.

キーワード 推奨
  1. (英) Alleles
  2. (英) Animals
  3. (英) Apoptosis
  4. (英) Blotting, Southern
  5. (英) Blotting, Western
  6. (英) Cell Differentiation
  7. (英) DNA-Binding Proteins
  8. (英) Epithelial Cells
  9. (英) Female
  10. (英) Gene Deletion
  11. (英) Genotype
  12. (英) Integrases
  13. (英) Lactation
  14. (英) Mammary Glands, Animal
  15. (英) Mice
  16. (英) Mice, Inbred C57BL
  17. (英) Mice, Knockout
  18. (英) Mice, Transgenic
  19. (英) Milk Proteins
  20. (英) Phenotype
  21. (英) Proto-Oncogene Proteins c-bcl-2
  22. (英) RNA
  23. (英) Receptors, Prolactin
  24. (英) Recombination, Genetic
  25. (英) Ribonucleases
  26. (英) STAT5 Transcription Factor
  27. (英) Spleen
  28. (英) Trans-Activators
  29. (英) Transgenes
  30. (英) Viral Proteins
  31. (英) bcl-X Protein
発行所 推奨
誌名 必須 Mechanisms of Development(The international Society of Developmental Biologists)
(pISSN: 0925-4773, eISSN: 1872-6356)
ISSN 任意 0925-4773
ISSN: 0925-4773 (pISSN: 0925-4773, eISSN: 1872-6356)
Title: Mechanisms of development
Title(ISO): Mech Dev
Publisher: John Wiley and Sons Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 109
必須 2
必須 281 293
都市 任意
年月日 必須 2001年 12月 初日
URL 任意
DOI 任意
PMID 任意 11731240    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) PublicationType: Journal Article