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著作: [青田 桂子]/[東 雅之]/Targeting TNF- suppresses the production of MMP-9 in human salivary gland cells./[Archives of Oral Biology]

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EID
284663
EOID
760312
Map
0
LastModified
2014年12月4日(木) 15:40:37
Operator
松井 栄里
Avail
TRUE
Censor
0
Owner
青田 桂子
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 青田 桂子([徳島大学.病院.診療科.歯科口腔外科.口腔内科])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 東 雅之
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Targeting TNF- suppresses the production of MMP-9 in human salivary gland cells.

副題 任意
要約 任意

(英) Tumour necrosis factor- (TNF-) is a pleiotropic cytokine that plays an essential role in inflammation and apoptosis. Our previous study suggested that TNF--induced activation of matrix metalloproteinase-9 (MMP-9) resulted in the destruction of acinar tissue in the salivary glands of patients with Sjögren's syndrome (SS) via disruption of the acinar cell-basement membrane. Recently, a wide array of biological agents has been designed to inhibit TNF, including etanercept and adalimumab. In this study, we demonstrate the suppressive effect of anti-TNF agents on TNF--induced MMP-9 production in NS-AV-AC, an immortalized human salivary gland acinar cell line. NS-AV-AC cells were treated with etanercept or adalimumab after TNF- treatment. MMP-9 production and enzymatic activity were, respectively, visualized by real-time PCR and ELISA assay, and evaluated by gelatin zymography, and apoptosis was evaluated by DNA fragmentation assay. TNF- induced the production of MMP-9 in NS-SV-AC cells. However, this production was greatly inhibited by treatment with etanercept or adalimumab. In addition, TNF--induced DNA fragmentation was prevented by treatment with etanercept or adalimumab. These results may indicate that anti-TNF agents would have therapeutic efficacy for preventing destruction of the acinar structure in the salivary glands of patients with SS.

キーワード 推奨
  1. (英) Acinar Cells
  2. (英) Antibodies, Monoclonal, Humanized
  3. (英) Antirheumatic Agents
  4. (英) Apoptosis
  5. (英) Cell Line
  6. (英) DNA Fragmentation
  7. (英) Electrophoresis, Polyacrylamide Gel
  8. (英) Enzyme-Linked Immunosorbent Assay
  9. (英) Gene Expression
  10. (英) Humans
  11. (英) Immunoglobulin G
  12. (英) Matrix Metalloproteinase 9
  13. (英) RNA, Messenger
  14. (英) Real-Time Polymerase Chain Reaction
  15. (英) Receptors, Tumor Necrosis Factor
  16. (英) Salivary Glands
  17. (英) Sjogren's Syndrome
  18. (英) Tumor Necrosis Factor-alpha
発行所 推奨
誌名 必須 Archives of Oral Biology([Elsevier])
(pISSN: 0003-9969, eISSN: 1879-1506)
ISSN 任意 1879-1506
ISSN: 0003-9969 (pISSN: 0003-9969, eISSN: 1879-1506)
Title: Archives of oral biology
Title(ISO): Arch Oral Biol
Publisher: Elsevier Ltd.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 58
必須 12
必須 1761 1768
都市 任意
年月日 必須 2013年 9月 21日
URL 任意
DOI 任意 10.1016/j.archoralbio.2013.09.004    (→Scopusで検索)
PMID 任意 24200302    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1016/j.archoralbio.2013.09.004

  2. (英) Article.ELocationID: S0003-9969(13)00294-X

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  5. (英) KeywordList.Keyword: Matrix metalloproteinase-9

  6. (英) KeywordList.Keyword: Salivary gland cells

  7. (英) KeywordList.Keyword: Tumour necrosis factor-