徳島大学 教育・研究者情報データベース(EDB)

1. 坂根 亜由子([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.生化学])

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2. (英) Abdallah Mahmoud Alamir Ahmed

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3. (英) Nakano Kiyoshi

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4. (英) Honda Kazufumi

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5. (英) Kitamura Toshio

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6. 井本 逸勢

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7. (英) Matsushita Natsuki

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8. 佐々木 卓也([徳島大学.事務局.研究・産学連携部])

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(英) Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.

(英) We previously showed that Rab13 and its effector protein, junctional Rab13-binding protein (JRAB)/molecules interacting with CasL-like 2 (MICAL-L2), regulate junctional development by modulating cell adhesion molecule transport and actin cytoskeletal reorganization in epithelial cells. Here, we investigated how JRAB regulates reorganization of the actin cytoskeleton in NIH3T3 fibroblasts, in an attempt to obtain novel insights into the mechanism of JRAB action. To this end, we expressed mutant proteins that adopt a constitutively open or closed state and then examined effect on cellular morphology of the resulting actin cytoskeletal reorganization. Expression of the JRABΔCT mutant (constitutively 'closed' state) induced stress fibers, whereas expression of the JRABΔCC mutant (constitutively 'open' state) caused cell spreading with membrane ruffles. Next, we identified the proteins involved in JRAB-induced rearrangement of actin cytoskeleton leading to morphological changes. In NIH3T3 cells expressing HA-JRABΔCC, filamin, an actin cross-linking protein, coimmunoprecipitated with HA-JRABΔCC. Expression of ASB2 induced degradation of all three filamin isoforms and inhibited the JRABΔCC-induced cell spreading. Consistent with our previous results, actinin-1/-4 were also immunoprecipitated with HA-JRABΔCC. However, actinin-1/-4 have no effect on the cell spreading regulated by JRABΔCC. These data suggest that JRAB contributes to the rearrangement of the actin cytoskeleton during cell spreading via filamins.

1. (英) Actinin
2. (英) Animals
3. (英) Cell Membrane
4. (英) Cytoskeletal Proteins
5. (英) Filamins
6. (英) HEK293 Cells
7. (英) Humans
8. (英) Mice
9. (英) Mutation
10. (英) NIH 3T3 Cells
11. (英) Protein Binding
12. (英) Protein Isoforms
13. (英) Protein Structure, Tertiary
14. (英) Stress Fibers

1. (英) Article.ELocationID: 10.1111/gtc.12078

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't