徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [中島 由紀子]/[稲垣 裕司]/[廣島 佑香]/[木戸 淳一]/[永田 俊彦]/Advanced glycation end-products enhance calcification in cultured rat dental pulp cells/[Journal of Endodontics]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
280273
EOID
748291
Map
0
LastModified
2014年9月17日(水) 09:59:11
Operator
松井 栄里
Avail
TRUE
Censor
0
Owner
木戸 淳一
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 中島 由紀子([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 稲垣 裕司([徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 廣島 佑香([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 木戸 淳一([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 永田 俊彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Advanced glycation end-products enhance calcification in cultured rat dental pulp cells

副題 任意
要約 任意

(英) Amorphous calcification frequently appears in dental pulp tissues of diabetic patients; however, its pathologic process has not been fully elucidated. We previously found that pulp stones and thickened predentin occurred more frequently in diabetic rats. Recent findings demonstrated that accumulation of advanced glycation end-products (AGE) might be involved in vascular calcification complicated with diabetes. The aim of this study was to determine the effect of AGE on calcified nodule formation by rat dental pulp cells in culture. Rat dental pulp cells and gingival fibroblasts were independently cultured with 50 and 100 μg/mL AGE. Alkaline phosphatase activity and calcified nodule formation were measured. Expressions of receptor for AGE, osteopontin (OPN), and osteocalcin (OCN) mRNA were determined by quantitative real-time polymerase chain reaction. Protein levels of OPN and OCN secreted in culture medium were quantified by enzyme-linked immunosorbent assay. AGE (50 and 100 μg/mL) markedly increased both alkaline phosphatase activity and calcified nodule formation in dental pulp cells (P < .01), whereas it did not affect those in gingival fibroblasts. Real-time polymerase chain reaction analysis revealed that AGE increased mRNA expressions of receptor for AGE, OPN, and OCN in dental pulp cells (P < .05). Enzyme-linked immunosorbent assay analysis revealed that the protein levels of OPN and OCN produced by dental pulp cells were higher in AGE-treated than in untreated cells (P < .05). AGE enhanced the calcification potentials of rat dental pulp cells, suggesting that it may stimulate pathologic calcification of diabetic dental pulp tissues.

キーワード 推奨
  1. (英) Alkaline Phosphatase
  2. (英) Animals
  3. (英) Cell Culture Techniques
  4. (英) Cells, Cultured
  5. (英) Dental Pulp
  6. (英) Dental Pulp Calcification
  7. (英) Fibroblasts
  8. (英) Gingiva
  9. (英) Glycosylation End Products, Advanced
  10. (英) Male
  11. (英) Osteocalcin
  12. (英) Osteopontin
  13. (英) Rats
  14. (英) Rats, Wistar
  15. (英) Receptors, Immunologic
  16. (英) Time Factors
発行所 推奨
誌名 必須 Journal of Endodontics([The American Association of Endodontics])
(pISSN: 0099-2399, eISSN: 1878-3554)
ISSN 任意 1878-3554
ISSN: 0099-2399 (pISSN: 0099-2399, eISSN: 1878-3554)
Title: Journal of endodontics
Title(ISO): J Endod
Publisher: Elsevier Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 39
必須 ---
必須 873 878
都市 任意
年月日 必須 2013年 1月 16日
URL 任意
DOI 任意 10.1016/j.joen.2012.11.027    (→Scopusで検索)
PMID 任意 23791254    (→Scopusで検索)
NAID 任意
WOS 任意 000321881100005
Scopus 任意 2-s2.0-84879412695
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1016/j.joen.2012.11.027

  2. (英) Article.ELocationID: S0099-2399(12)01085-0

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't