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著作: Ogawa, Motohiko/[内山 恒夫]/Satoh, Masaaki/Ando, Shuji/Decontamination of mycoplasma-contaminated Orientia tsutsugamushi strains by repeating passages through cell cultures with antibiotics/[BMC Microbiology]

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EID
269013
EOID
971124
Map
0
LastModified
2020年1月31日(金) 13:39:17
Operator
三木 ちひろ
Avail
TRUE
Censor
0
Owner
[副研究部長]/[徳島大学.大学院医歯薬学研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Ogawa, Motohiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 内山 恒夫
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Satoh, Masaaki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Ando, Shuji
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Decontamination of mycoplasma-contaminated Orientia tsutsugamushi strains by repeating passages through cell cultures with antibiotics

副題 任意
要約 任意

(英) Mycoplasmas-contamination of Orientia tsutsugamushi, one of the obligated intracellular bacteria, is a very serious problem in in vitro studies using cell cultures because mycoplasmas have significant influence on the results of scientific studies. Only a recommended decontamination method is to passage the contaminated O. tsutsugamushi strains through mice to eliminate only mycoplasmas under influence of their immunity. However, this method sometimes does not work especially for low virulent strains of O. tsutsugamushi which are difficult to propagate in mice. In this study, we tried to eliminate mycoplasmas contaminants from both high virulent and low virulent strains of the contaminated O. tsutsugamushi by repeating passage through cell cultures with antibiotics in vitro. We cultured a contaminated, high virulent strain of O. tsutsugamushi using a mouse lung fibroblasts cell line, L-929 cell in the culture medium containing lincomycin at various concentrations and repeated passages about every seven days. At the passage 5 only with 10 μg/ml of lincomycin, we did not detect mycoplasmas by two PCR based methods whereas O. tsutsugamushi continued good growth. During following four passages without lincomycin, mycoplasmas did not recover. These results suggested that mycoplasmas were completely eliminated from the high virulent strain of O. tsutsugamushi. Furthermore, by the same procedures with 10 μg/ml of lincomycin, we also eliminated mycoplasmas from a contaminated, low virulent strain of O. tsutsugamushi. Our additional assay showed that 50 μg/ml of lyncomycin did not inhibit the growth of O. tsutsugamushi, although MICs of many mycoplasmas contaminants were less than 6 μg/ml as shown previously. Our results showed an alternative method to eliminate mycoplasmas from the contaminated O. tsutsugamushi strains in place of in vivo passage through mice. Especially this notable method works for the decontamination not only from the high virulent strain also from the low virulent strain of O. tsutsugamushi. For further elimination, lincomycin at the limit concentration, which does not inhibit the growth of O. tsutsugamushi, can possibly eliminate most mycoplasmas from contaminated O. tsutsugamushi strains.

キーワード 推奨
  1. (英) Animals
  2. (英) Anti-Bacterial Agents
  3. (英) Cell Culture Techniques
  4. (英) Cell Line
  5. (英) Culture Media
  6. (英) Decontamination
  7. (英) Fibroblasts
  8. (英) Lincomycin
  9. (英) Mice
  10. (英) Mycoplasma
  11. (英) Orientia tsutsugamushi
  12. (英) Serial Passage
発行所 推奨
誌名 必須 BMC Microbiology([BioMed Central Ltd.])
(eISSN: 1471-2180)
ISSN 任意 1471-2180
ISSN: 1471-2180 (eISSN: 1471-2180)
Title: BMC microbiology
Title(ISO): BMC Microbiol
Publisher: BioMed Central
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 13
必須 32
必須 ---
都市 任意
年月日 必須 2013年 2月 8日
URL 任意
DOI 任意 10.1186/1471-2180-13-32    (→Scopusで検索)
PMID 任意 23394970    (→Scopusで検索)
CRID 任意
WOS 任意 000315713000001
Scopus 任意 2-s2.0-84873436719
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1186/1471-2180-13-32

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC3598641