著作: [髙田 香]/[富山 芳信]/[田中 克哉]/[大下 修造]/Cardioprotective effects of hyperkalemia during smulated ischemia/reperfusion in neonatal rat cardiomyocytes - Preservation of Na+/K+-ATPase activity -/[The Journal of Medical Investigation : JMI]
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種別 | 必須 | 学術論文(審査論文) | |||
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言語 | 必須 | 英語 | |||
招待 | 推奨 | ||||
審査 | 推奨 | Peer Review | |||
カテゴリ | 推奨 | ||||
共著種別 | 推奨 | ||||
学究種別 | 推奨 | 博士後期課程学生による研究報告 | |||
組織 | 推奨 | ||||
著者 | 必須 | ||||
題名 | 必須 |
(英) Cardioprotective effects of hyperkalemia during smulated ischemia/reperfusion in neonatal rat cardiomyocytes - Preservation of Na+/K+-ATPase activity - |
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副題 | 任意 | ||||
要約 | 任意 |
(英) Hyperkalemia has multimodal effects on myocardial protection during ischemia/reperfusion. The preservation of Na(+)/K(+)-ATPase activity induced by hyperkalemia may have critical impact on myocardial protection. To elucidate the roles of hyperkalemia (16 mM) and Na(+)/K(+)-ATPase inhibition (100 µM ouabain) in myocardial protection during simulated ischemia (5 mM NaCN and 5.5 mM 2-deoxyglucose)/reperfusion, we measured loss of membrane integrity and bleb formation using a vital dye calcein AM in cultured neonatal rat cardiomyocytes. The control perfusate was switched to treatment solution for 15 min, followed by reperfusion for 30 min. In a second set of experiments, myocardial excitability and diastolic intracellular calcium ion concentration ([Ca(2+)]i) were measured during a 45-min treatment using a calcium-sensitive fluorescent dye fluo-4 AM. Simulated ischemia/reperfusion under ouabain treatment induced loss of membrane integrity, which was suppressed by hyperkalemia. Simulated ischemia/reperfusion induced bleb formation, which was accelerated by ouabain. Hyperkalemia delayed and inhibited the increase in diastolic [Ca(2+)]i induced by simulated ischemia. Furthermore, hyperkalemia almost completely inhibited the effects of ouabain on the diastolic [Ca(2+)]i during ischemia. These results suggest that hyperkalemia during ischemia is cardioprotective against ischemia/reperfusion insults and that hyperkalemia inhibits the effects of ouabain during ischemia. |
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キーワード | 推奨 |
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発行所 | 推奨 | ||||
誌名 | 必須 |
The Journal of Medical Investigation : JMI([徳島大学.医学部])
(pISSN: 1343-1420, eISSN: 1349-6867)
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巻 | 必須 | 60 | |||
号 | 必須 | 1,2, | |||
頁 | 必須 | 66 76 | |||
都市 | 任意 | ||||
年月日 | 必須 | 2013年 2月 初日 | |||
URL | 任意 | ||||
DOI | 任意 | 10.2152/jmi.60.66 (→Scopusで検索) | |||
PMID | 任意 | 23614914 (→Scopusで検索) | |||
CRID | 任意 | ||||
WOS | 任意 | ||||
Scopus | 任意 | ||||
機関リポジトリ | 111301 | ||||
評価値 | 任意 | ||||
被引用数 | 任意 | ||||
指導教員 | 推奨 | ||||
備考 | 任意 |
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