徳島大学 教育・研究者情報データベース(EDB)

1. (英) Eskander N. Ramez

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2. (英) Randall M. Leslie

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3. 酒井 紀典([徳島大学.病院.中央診療施設等.高度先進整形外科診療部])

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4. (英) Guo Yi

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5. (英) Hoang Bang

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6. (英) Zi Xiaolin

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(英) Flavokawain B, a novel, naturally occurring chalcone, exhibits robust apoptotic effects and induces G2/M arrest of a uterine leiomyosarcoma cell line.

(英) To examine the effects of flavokawain B (FKB), a novel kava chalcone, on the growth of uterine leiomyosarcoma (LMS) cells and investigated its utility in the treatment of uterine LMS. Uterine leiomyosarcoma (SK-LMS-1), endometrial adenocarcinoma (ECC-1) and the non-malignant, human endometrium fibroblast-like (T-HESC) cell lines were cultured and treated with different concentrations of FKB. Cell viability was determined by MTT assays and the IC(50) was estimated. Fluorescent-activated cell sorting (FACS) analysis of apoptosis and cell cycle was performed. Real-time reverse-transcription polymerase chain reaction and western blot analysis were utilized to evaluate differences in the expression of apoptotic markers. FKB preferentially inhibited the growth of SK-LMS-1 and ECC-1 cells compared to T-HESC control cells. FKB significantly increased both early and late apoptosis in SK-LMS-1 and ECC-1 cells relative to control. Cell cycle analysis illustrated an increase in the G2/M fraction in treated cell lines relative to control. Furthermore, FKB induced the expression of pro-apoptotic death receptor 5 (DR5), Bim, and Puma, and decreased expression of an inhibitor of apoptosis, survivin. FKB also acted synergistically when combined with docetaxel and gemcitabine (combination index = 0.260). FKB treatment results in cell cycle arrest and a robust induction of apoptosis in SK-LMS-1 and ECC-1 cell lines. This natural product deserved further investigation as a potential therapeutic agent in the treatment of uterine LMS.

1. (英) Antineoplastic Agents
2. (英) Apoptosis
3. (英) Cell Line, Tumor
4. (英) Deoxycytidine
5. (英) Drug Evaluation, Preclinical
6. (英) Drug Synergism
7. (英) Female
8. (英) Fibroblasts
9. (英) Flavonoids
10. (英) G2 Phase Cell Cycle Checkpoints
11. (英) Gene Expression Regulation, Neoplastic
12. (英) Humans
13. (英) Inhibitor of Apoptosis Proteins
14. (英) Leiomyosarcoma
15. (英) Phytotherapy
16. (英) Plant Extracts
17. (英) Taxoids
18. (英) Uterine Neoplasms

1. (英) Article.ELocationID: 10.1111/j.1447-0756.2011.01841.x

2. (英) Article.Affiliation: Departments of Obstetrics and Gynecology, University of California Irvine, Orange, CA, USA.

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural

5. (英) OtherID: NIHMS343335

6. (英) OtherID: PMC3405181