著作: Hyodo Kiwamu/Mine Akira/[谷口 貴子]/Kaido Masanori/Mise Kazuyuki/[谷口 寿章]/Okuno Tetsuro/ADP ribosylation factor 1 plays an essential role in the replication of a plant RNA virus./[Journal of Virology]
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削除する| 種別 | 必須 | 学術論文(審査論文) | |||
|---|---|---|---|---|---|
| 言語 | 必須 | 英語 | |||
| 招待 | 推奨 | ||||
| 審査 | 推奨 | ||||
| カテゴリ | 推奨 | ||||
| 共著種別 | 推奨 | ||||
| 学究種別 | 推奨 | ||||
| 組織 | 推奨 | ||||
| 著者 | 必須 | ||||
| 題名 | 必須 |
(英) ADP ribosylation factor 1 plays an essential role in the replication of a plant RNA virus. |
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| 副題 | 任意 | ||||
| 要約 | 任意 |
(英) Eukaryotic positive-strand RNA viruses replicate using the membrane-bound replicase complexes, which contain multiple viral and host components. Virus infection induces the remodeling of intracellular membranes. Virus-induced membrane structures are thought to increase the local concentration of the components that are required for replication and provide a scaffold for tethering the replicase complexes. However, the mechanisms underlying virus-induced membrane remodeling are poorly understood. RNA replication of red clover necrotic mosaic virus (RCNMV), a positive-strand RNA plant virus, is associated with the endoplasmic reticulum (ER) membranes, and ER morphology is perturbed in RCNMV-infected cells. Here, we identified ADP ribosylation factor 1 (Arf1) in the affinity-purified RCNMV RNA-dependent RNA polymerase fraction. Arf1 is a highly conserved, ubiquitous, small GTPase that is implicated in the formation of the coat protein complex I (COPI) vesicles on Golgi membranes. Using in vitro pulldown and bimolecular fluorescence complementation analyses, we showed that Arf1 interacted with the viral p27 replication protein within the virus-induced large punctate structures of the ER membrane. We found that inhibition of the nucleotide exchange activity of Arf1 using the inhibitor brefeldin A (BFA) disrupted the assembly of the viral replicase complex and p27-mediated ER remodeling. We also showed that BFA treatment and the expression of dominant negative Arf1 mutants compromised RCNMV RNA replication in protoplasts. Interestingly, the expression of a dominant negative mutant of Sar1, a key regulator of the biogenesis of COPII vesicles at ER exit sites, also compromised RCNMV RNA replication. These results suggest that the replication of RCNMV depends on the host membrane traffic machinery. |
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| キーワード | 推奨 |
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| 発行所 | 推奨 | ||||
| 誌名 | 必須 |
Journal of Virology([アメリカ微生物学会])
(pISSN: 0022-538X, eISSN: 1098-5514)
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| 巻 | 必須 | 87 | |||
| 号 | 必須 | 1 | |||
| 頁 | 必須 | 163 176 | |||
| 都市 | 任意 | ||||
| 年月日 | 必須 | 2013年 1月 初日 | |||
| URL | 任意 | ||||
| DOI | 任意 | 10.1128/JVI.02383-12 (→Scopusで検索) | |||
| PMID | 任意 | 23097452 (→Scopusで検索) | |||
| NAID | 任意 | ||||
| WOS | 任意 | ||||
| Scopus | 任意 | ||||
| 評価値 | 任意 | ||||
| 被引用数 | 任意 | ||||
| 指導教員 | 推奨 | ||||
| 備考 | 任意 |
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