徳島大学 教育・研究者情報データベース(EDB)

1. 木戸 淳一

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2. 阿部 佳織

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3. (英) Yatsushiro Shouki / (日) 八代 聖基 / (読) やつしろ しょうき

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4. 板東 美香([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])

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5. 廣島 佑香([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])

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6. 永田 俊彦

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7. 大家 利彦(産業技術総合研究所健康工学研究センター)

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8. 田中 正人((独)産業技術総合研究所 健康工学研究センター)

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9. 片岡 正俊

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(英) Determination of calprotectin in gingival crevicular fluid by immunoassay on a microchip

(英) Gingival crevicular fluid (GCF) contains calprotectin, which appears to be a useful biomarker for periodontal diseases because of its high level in GCF from periodontally diseased pockets. To determine calprotectin in GCF that has a very small volume, sandwich enzyme-linked immunosorbent assay (ELISA) on a microchip was performed and its utility was estimated. Anti-calprotectin primary antibody was discharged on a microchip using a piezoelectric inkjet printing system. Calprotectin standard and calprotectin in GCF samples from eleven subjects were determined by the ELISA method with the prepared microchip and their values were compared with those obtained by conventional ELISA. Using the ELISA on a microchip, a reasonable standard curve of calprotectin protein (1.56-100 ng/mL) was obtained. Calprotectin in GCF samples was quantified and showed reasonable values in accordance with the condition of periodontal diseases. The values determined by the microchip method and conventional ELISA showed a significant linear relationship (R(2)=0.981). Calprotectin in GCF was determined using the ELISA on a microchip with high efficiency and this ELISA method for calprotectin determination may become a useful method for diagnosing periodontal diseases.

1. (英) Aged
2. (英) Antibodies, Immobilized
3. 校正(calibration)
4. (英) Enzyme-Linked Immunosorbent Assay
5. (英) Female
6. (英) Gingival Crevicular Fluid
7. (英) Humans
8. (英) Lab-On-A-Chip Devices
9. (英) Leukocyte L1 Antigen Complex
10. (英) Linear Models
11. (英) Male
12. (英) Microfluidic Analytical Techniques
13. (英) Middle Aged
14. (英) Periodontal Diseases
15. (英) Reference Standards
16. (英) Reproducibility of Results
17. (英) Sensitivity and Specificity

1. (英) Article.ELocationID: 10.1016/j.clinbiochem.2012.05.009

2. (英) Article.ELocationID: S0009-9120(12)00242-1

3. (英) Article.Affiliation: Department of Periodontology and Endodontology, Institute of Health Biosciences, The University of Tokushima Graduate School, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. kido@dent.tokushima-u.ac.jp

4. (英) Article.PublicationTypeList.PublicationType: Journal Article

5. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't