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著作: [高石 和美]/[北畑 洋]/[川人 伸次]/Local anesthetics inhibit nitric oxide production and l-arginine uptake in cultured bovine aortic endothelial cells./[European Journal of Pharmacology]

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EID
264180
EOID
796593
Map
0
LastModified
2015年9月7日(月) 15:41:02
Operator
大家 隆弘
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TRUE
Censor
0
Owner
北畑 洋
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 高石 和美([徳島大学.病院.診療科.歯科口腔外科.歯科麻酔科]/[徳島大学.歯学部.歯学科.口腔疾患系臨床歯学講座.歯科麻酔学 (歯科麻酔科学講座)])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 北畑 洋([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯科麻酔科学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 川人 伸次([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).地域医療人材育成(医学域)])
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Local anesthetics inhibit nitric oxide production and l-arginine uptake in cultured bovine aortic endothelial cells.

副題 任意
要約 任意

(英) Previous studies have shown that local anesthetics have various effects on nitric oxide (NO) production, but the mechanisms remain unclear. The purpose of this study was to evaluate the effects of local anesthetics on NO production and 2-amino-5-guanidinopentanoic acid (l-arginine) uptake in one cell line. Cultured bovine aortic endothelial cells (BAEC) were stimulated with bradykinin and/or acetylcholine to activate endothelial NO synthase (NOS). BAEC were also incubated with interleukin-1β and lipopolysaccharide to stimulate inducible NOS. NO production was measured with the rapid spectrophotometric method, and l-arginine uptake was measured with high performance liquid chromatography. To assess the effects of local anesthetics, NO production and l-arginine uptake were measured in the presence or absence of procaine or lidocaine. NO was produced in BAEC stimulated with bradykinin and acetylcholine or interleukin-1β and lipopolysaccharide, but NO production was not affected by the addition of superoxide dismutase. In the cells stimulated with bradykinin and acetylcholine, 10 μM each of procaine and lidocaine significantly inhibited NO production by 35% and 20%, respectively. In the cells incubated with interleukin-1ß and lipopolysaccharide, the same quantities of procaine and lidocaine significantly inhibited NO production by 15% and 10%, respectively. Both procaine and lidocaine significantly suppressed l-arginine uptake in BAEC stimulated with either bradykinin/acetylcholine or interleukin-1β/lipopolysaccharide. It is suggested that inhibitory effects of procaine and lidocaine on NO production are partially due to suppression of l-arginine uptake.

キーワード 推奨
  1. (英) Anesthetics, Local
  2. (英) Animals
  3. (英) Aorta
  4. (英) Arginine
  5. (英) Cattle
  6. (英) Cells, Cultured
  7. (英) Endothelial Cells
  8. (英) Lidocaine
  9. (英) Nitric Oxide
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] 一酸化窒素 (nitric oxide)
  10. (英) Nitric Oxide Synthase Type II
  11. (英) Nitric Oxide Synthase Type III
  12. (英) Procaine
発行所 推奨
誌名 必須 European Journal of Pharmacology([Elsevier])
(pISSN: 0014-2999, eISSN: 1879-0712)
ISSN 任意 1879-0712
ISSN: 0014-2999 (pISSN: 0014-2999, eISSN: 1879-0712)
Title: European journal of pharmacology
Title(ISO): Eur. J. Pharmacol.
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 704
必須 1-3
必須 58 63
都市 任意
年月日 必須 2013年 2月 24日
URL 任意
DOI 任意 10.1016/j.ejphar.2013.02.014    (→Scopusで検索)
PMID 任意 23461848    (→Scopusで検索)
NAID 任意
WOS 任意 000317184900008
Scopus 任意 2-s2.0-84874940599
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1016/j.ejphar.2013.02.014

  2. (英) Article.ELocationID: S0014-2999(13)00110-6

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't