徳島大学 教育・研究者情報データベース(EDB)

1. 高石 和美([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯科麻酔科学]/[徳島大学.病院.診療科.歯科口腔外科.歯科麻酔科]/[徳島大学.歯学部.歯学科.口腔疾患系臨床歯学講座.歯科麻酔科学])

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2. 北畑 洋

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3. 川人 伸次([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯科麻酔科学])

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(英) Local anesthetics inhibit nitric oxide production and l-arginine uptake in cultured bovine aortic endothelial cells.

(英) Previous studies have shown that local anesthetics have various effects on nitric oxide (NO) production, but the mechanisms remain unclear. The purpose of this study was to evaluate the effects of local anesthetics on NO production and 2-amino-5-guanidinopentanoic acid (l-arginine) uptake in one cell line. Cultured bovine aortic endothelial cells (BAEC) were stimulated with bradykinin and/or acetylcholine to activate endothelial NO synthase (NOS). BAEC were also incubated with interleukin-1β and lipopolysaccharide to stimulate inducible NOS. NO production was measured with the rapid spectrophotometric method, and l-arginine uptake was measured with high performance liquid chromatography. To assess the effects of local anesthetics, NO production and l-arginine uptake were measured in the presence or absence of procaine or lidocaine. NO was produced in BAEC stimulated with bradykinin and acetylcholine or interleukin-1β and lipopolysaccharide, but NO production was not affected by the addition of superoxide dismutase. In the cells stimulated with bradykinin and acetylcholine, 10 μM each of procaine and lidocaine significantly inhibited NO production by 35% and 20%, respectively. In the cells incubated with interleukin-1ß and lipopolysaccharide, the same quantities of procaine and lidocaine significantly inhibited NO production by 15% and 10%, respectively. Both procaine and lidocaine significantly suppressed l-arginine uptake in BAEC stimulated with either bradykinin/acetylcholine or interleukin-1β/lipopolysaccharide. It is suggested that inhibitory effects of procaine and lidocaine on NO production are partially due to suppression of l-arginine uptake.

1. (英) Anesthetics, Local
2. (英) Animals
3. (英) Aorta
4. (英) Arginine
5. (英) Cattle
6. (英) Cells, Cultured
7. (英) Endothelial Cells
8. (英) Lidocaine
9. (英) Nitric Oxide
10. (英) Nitric Oxide Synthase Type II
11. (英) Nitric Oxide Synthase Type III
12. (英) Procaine

1. (英) Article.ELocationID: 10.1016/j.ejphar.2013.02.014

2. (英) Article.ELocationID: S0014-2999(13)00110-6

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't