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著作: [山口 鉄生]/Suzuki Takayoshi/Arai Hideaki/Tanabe Shihori/Atomi Yoriko/Continuous mild heat stress induces differentiation of mammalian myoblasts, shifting fiber type from fast to slow./[American Journal of Physiology, Cell Physiology]

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EID
262348
EOID
689508
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0
LastModified
2013年6月4日(火) 21:02:10
Operator
大家 隆弘
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Owner
山口 鉄生
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 山口 鉄生([徳島大学.大学院社会産業理工学研究部.社会総合科学域.人間科学系.スポーツ健康分野]/[徳島大学.総合科学部.社会総合科学科.心身健康コース])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Suzuki Takayoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Arai Hideaki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Tanabe Shihori
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Atomi Yoriko
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Continuous mild heat stress induces differentiation of mammalian myoblasts, shifting fiber type from fast to slow.

副題 任意
要約 任意

(英) Local hyperthermia has been widely used as physical therapy for a number of diseases such as inflammatory osteoarticular disorders, tendinitis, and muscle injury. Local hyperthermia is clinically applied to improve blood and lymphatic flow to decrease swelling of tissues (e.g., skeletal muscle). As for muscle repair following injury, the mechanisms underlying the beneficial effects of hyperthermia-induced muscle repair are unknown. In this study, we investigated the direct effects of continuous heat stress on the differentiation of cultured mammalian myoblasts. Compared with control cultures grown at 37 degrees C, incubation at 39 degrees C (continuous mild heat stress; CMHS) enhanced myotube diameter, whereas myotubes were poorly formed at 41 degrees C by primary human skeletal muscle culture cells, human skeletal muscle myoblasts (HSMMs), and C2C12 mouse myoblasts. In HSMMs and C2C12 cells exposed to CMHS, mRNA and protein levels of myosin heavy chain (MyHC) type I were increased compared with the control cultures. The mRNA level of MyHC IIx was unaltered in HSMMs and decreased in C2C12 cells, compared with cells that were not exposed to heat stress. These results indicated a fast-to-slow fiber-type shift in myoblasts. We also examined upstream signals that might be responsible for the fast-to-slow shift of fiber types. CMHS enhanced the mRNA and protein levels of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha in HSMMS and C2C12 cells but not the activities of MAPKs (ERK1/2 and p38 MAPK) in HSMMs and C2C12 cells. These data suggest that CMHS induces a fast-to-slow fiber-type shift of mammalian myoblasts through PGC-1alpha.

キーワード 推奨
  1. (英) Actins
  2. (英) Animals
  3. 細胞分化(cell differentiation)
  4. (英) Cells, Cultured
  5. (英) Genes, Reporter
  6. (英) Green Fluorescent Proteins
  7. (英) Heat-Shock Proteins
  8. (英) Hot Temperature
  9. (英) Humans
  10. (英) Mammals
  11. (英) Mice
  12. (英) Mitogen-Activated Protein Kinase 3
  13. (英) Muscle Fibers, Fast-Twitch
  14. (英) Muscle Fibers, Skeletal
  15. (英) Muscle Fibers, Slow-Twitch
  16. (英) Myoblasts
  17. (英) Myosin Heavy Chains
  18. (英) Protein Isoforms
  19. (英) RNA, Messenger
  20. (英) Reverse Transcriptase Polymerase Chain Reaction
  21. (英) Transcription Factors
  22. (英) p38 Mitogen-Activated Protein Kinases
発行所 推奨
誌名 必須 American Journal of Physiology, Cell Physiology([アメリカ生理学会])
(pISSN: 0363-6143, eISSN: 1522-1563)
ISSN 任意 1522-1563
ISSN: 0363-6143 (pISSN: 0363-6143, eISSN: 1522-1563)
Title: American journal of physiology. Cell physiology
Title(ISO): Am J Physiol Cell Physiol
Publisher: American Physiological Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 298
必須 1
必須 C140 8
都市 任意
年月日 必須 2009年 7月 15日
URL 任意
DOI 任意 10.1152/ajpcell.00050.2009    (→Scopusで検索)
PMID 任意 19605738    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.ELocationID: 10.1152/ajpcell.00050.2009

  2. (英) Article.Affiliation: Deptarment of Life Sciences, The Graduate School of Arts and Sciences, The Univ. of Tokyo, 3-8-1, Komaba, Meguro-ku, Tokyo 153-8902, Japan. tetsuo-yama@abeam.ocn.ne.jp

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article