徳島大学 教育・研究者情報データベース(EDB)

1. 池田 康将([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.薬理学]/[徳島大学.医学部.医学科.病態情報医学講座.薬理学分野]/[徳島大学.医学研究科.医学専攻.病態情報医学講座]/[徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.薬理学])

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2. (英) Tajima Soichiro

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3. 石澤 有紀([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.臨床薬理学]/->個人[井澤 有紀])

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4. 木平 孝高

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5. 石澤 啓介([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.臨床薬理学])

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6. 吉田 守美子

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7. 粟飯原 賢一([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).実践地域診療・医科学])

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8. 土屋 浩一郎([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品機能生化学]/[徳島大学.医学部.医学科.病態情報医学講座])

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9. 玉置 俊晃

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(英) Bovine milk-derived lactoferrin exerts proangiogenic effects in an Src-Akt-eNOS-dependent manner in response to ischemia

(英) Lactoferrin (LF) exerts a variety of biological effects, including the promotion of angiogenesis by increasing the expression of angiogenesis-related genes and reducing blood pressure via a nitric oxide-dependent mechanism. In this study, we investigated the effects of LF on angiogenesis using C57BL/6J mice that received daily unilateral treatment with or without bovine milk-derived LF (bLF) after unilateral hindlimb surgery. The analysis of laser speckle blood flow showed that bLF treatment promoted blood flow recovery in response to ischemic hindlimb. The capillary density of ischemic adductor muscles and the phosphorylation of Src, Akt, and endothelial nitric oxide synthase (eNOS) were also significantly higher in bLF-treated mice than in vehicle-treated mice. Furthermore, bLF increased the phosphorylation levels of Src, Akt, and eNOS in in vitro experiments using human aortic endothelial cells. The action of bLF on eNOS phosphorylation was abolished by both LY294002, a phosphatidylinositol 3-kinase inhibitor, and 4-amino-5-(4-chlorophenyl)-7-(dimethylethyl)pyrazolo [3,4-d]pyrimidine (PP2), an Src inhibitor. Similarly, bLF-induced acceleration of tube formation, cell proliferation, and cell migration in human aortic endothelial cells were inhibited by LY294002 or PP2. Thus, bLF promotes vascular endothelial cell function via an Src Akt eNOS-dependent pathway, thereby contributing to revascularization in response to ischemia.

1. (英) Animals
2. (英) Aorta
3. (英) Cell Movement
4. (英) Cell Proliferation
5. (英) Cells, Cultured
6. (英) Endothelial Cells
7. (英) Endothelium, Vascular
8. (英) Enzyme Activation
9. (英) Hindlimb
10. (英) Humans
11. (英) Ischemia
12. (英) Lactoferrin
13. (英) Laser-Doppler Flowmetry
14. (英) Mice
15. (英) Mice, Inbred C57BL
16. (英) Milk
17. (英) Neovascularization, Physiologic
18. (英) Nitric Oxide Synthase Type III
19. (英) Phosphorylation
20. (英) Proto-Oncogene Proteins c-akt
21. (英) Reperfusion Injury
22. (英) Signal Transduction
23. (英) src-Family Kinases

1. (英) Article.ELocationID: 10.1097/FJC.0b013e318287d526

2. (英) Article.PublicationTypeList.PublicationType: In Vitro

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't