徳島大学 教育・研究者情報データベース(EDB)

1. 鳥羽 博明([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.看護学系.臨床腫瘍医療学])

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2. 先山 正二

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3. 監﨑 孝一郎

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4. 川上 行奎

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5. (英) Uyama Koh

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6. 坂東 良美([徳島大学.病院.診療科.病理診断科])

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7. 丹黒 章

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(英) Implantation of fetal rat lung fragments into bleomycin-induced pulmonary fibrosis

(英) Pulmonary fibrosis is a life-threatening disease that results in progressive respiratory failure. We have suggested the possibility of fetal lung tissue as an option for further investigation into lung regeneration. The objective was to prove whether fetal lung fragments can survive and differentiate in fibrotic lung. Lewis rats were administered bleomycin and used as recipients after 3 or 4 weeks. Day 17 fetal lung tissue from green fluorescent protein Lewis rats was used as donor material. Donor lungs were removed, cut into small pieces, and implanted into the recipients' left lung. The recipients received cyclosporin to prevent immune response to green fluorescent protein and were killed after 1, 2, 4, 8, and 12 weeks and histologically evaluated. Furthermore, the expression of thyroid transcription factor-1 and Clara cell secretory protein in the implanted fetal lung tissue was immunohistologically evaluated. Fibrotic changes were recognized for a long period of time in the recipient lungs. The implanted fetal lung fragments could be clearly distinguished from recipient lungs because of the luminescence of grafts. Fetal lung fragments could survive in the recipient lungs with fibrotic changes. The air spaces of implanted fetal lungs were narrow at 1 and 2 weeks but expanded with the passage of time. The connection between the recipient lung and the implanted fetal lung was recognized, particularly in the peripheral grafts. The expression patterns of thyroid transcription factor-1 and Clara cell secretory protein in implanted lungs resembled those in the process of normal lung morphogenesis. Fetal rat lung fragments could survive and differentiate in bleomycin-induced completely fibrotic lung.

1. (英) Animals
2. (英) Animals, Genetically Modified
3. (英) Bleomycin
4. 細胞分化(cell differentiation)
5. (英) Disease Models, Animal
6. (英) Female
7. (英) Graft Survival
8. (英) Green Fluorescent Proteins
9. 免疫組織化学(immunohistochemistry)
10. (英) Lung
11. (英) Lung Transplantation
12. (英) Male
13. (英) Nuclear Proteins
14. 肺線維症(pulmonary fibrosis)
15. (英) Rats
16. (英) Rats, Inbred Lew
17. 再生(regeneration)
18. (英) Time Factors
19. (英) Transcription Factors
20. (英) Uteroglobin

1. (英) Article.ELocationID: 10.1016/j.jtcvs.2012.01.002

2. (英) Article.Affiliation: Department of Thoracic and Endocrine Surgery, The University of Tokushima, Kuramoto-cho, Tokushima, Japan.

3. (英) Article.PublicationTypeList.PublicationType: Journal Article