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著作: Mistry S. Devendra/[堤 理恵]/Fernandez Marina/Sharma Shweta/Cardenas A. Steven/Lawson A. Mark/Webster J. G. Nicholas/Gonadotropin-releasing hormone pulse sensitivity of follicle-stimulating hormone-beta gene is mediated by differential expression of positive regulatory activator protein 1 factors and corepressors SKIL and TGIF1./[Molecular Endocrinology]

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EID
254564
EOID
655767
Map
0
LastModified
2012年8月29日(水) 18:03:43
Operator
大家 隆弘
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TRUE
Censor
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Owner
堤 理恵
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種別 必須
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Mistry S. Devendra
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 堤 理恵([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.代謝栄養学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Fernandez Marina
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Sharma Shweta
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Cardenas A. Steven
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Lawson A. Mark
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Webster J. G. Nicholas
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Gonadotropin-releasing hormone pulse sensitivity of follicle-stimulating hormone-beta gene is mediated by differential expression of positive regulatory activator protein 1 factors and corepressors SKIL and TGIF1.

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要約 任意

(英) Gonadotropin synthesis and release is dependent on pulsatile stimulation by the hypothalamic neuropeptide GnRH. Generally, slow GnRH pulses promote FSH production, whereas rapid pulses favor LH, but the molecular mechanism underlying this pulse sensitivity is poorly understood. In this study, we developed and tested a model for FSHβ regulation in mouse LβT2 gonadotropes. By mining a previous microarray data set, we found that mRNA for positive regulators of Fshb expression, such as Fos and Jun, were up-regulated at slower pulse frequencies than a number of potential negative regulators, such as the corepressors Skil, Crem, and Tgif1. These latter corepressors reduced Fshb promoter activity whether driven by transfection of individual transcription factors or by treatment with GnRH and activin. Overexpression of binding or phosphorylation-defective ski-oncogene-like protein (SKIL) and TG interacting factor (TGIF1) mutants, however, failed to repress Fshb promoter activity. Knockdown of the endogenous repressors SKIL and TGIF1, but not cAMP response element-modulator, increased Fshb promoter activity driven by constant GnRH or activin. Chromatin immunoprecipitation analysis showed that FOS, SKIL, and TGIF1 occupy the FSHβ promoter in a cyclical manner after GnRH stimulation. Overexpression of corepressors SKIL or TGIF1 repressed induction of the Fshb promoter at the slow GnRH pulse frequency but had little effect at the fast pulse frequency. In contrast, knockdown of endogenous SKIL or TGIF1 selectively increased Fshb mRNA at the fast GnRH pulse frequency. Therefore, we propose a potential mechanism by which production of gonadotropin Fshb is modulated by positive transcription factors and negative corepressors with different pulse sensitivities.

キーワード 推奨
  1. (英) Animals
  2. (英) Co-Repressor Proteins
  3. (英) Cyclic AMP Response Element Modulator
  4. (英) Follicle Stimulating Hormone, beta Subunit
  5. (英) Gene Expression Regulation
  6. (英) Gene Knockdown Techniques
  7. (英) Gonadotropin-Releasing Hormone
  8. (英) Homeodomain Proteins
  9. (英) Kinetics
  10. (英) Mice
  11. (英) Models, Biological
  12. (英) Mutation
  13. (英) Promoter Regions, Genetic
  14. (英) Protein Subunits
  15. (英) Proto-Oncogene Proteins
  16. (英) Proto-Oncogene Proteins c-fos
  17. (英) Proto-Oncogene Proteins c-jun
  18. (英) RNA, Messenger
  19. (英) Rats
  20. (英) Repressor Proteins
  21. (英) Transcription Factor AP-1
発行所 推奨
誌名 必須 Molecular Endocrinology([The Endocrine Society])
(pISSN: 0888-8809, eISSN: 1944-9917)
ISSN 任意 1944-9917
ISSN: 0888-8809 (pISSN: 0888-8809, eISSN: 1944-9917)
Title: Molecular endocrinology (Baltimore, Md.)
Title(ISO): Mol Endocrinol
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 25
必須 8
必須 1387 1403
都市 任意
年月日 必須 2011年 6月 9日
URL 任意
DOI 任意 10.1210/me.2011-0032    (→Scopusで検索)
PMID 任意 21659477    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Medical Research Service, Veterans Affairs San Diego Healthcare System, San Diego, California 92161, USA.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, N.I.H., Extramural

  4. (英) OtherID: PMC3146254