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著作: Magari Masaki/[西川 裕美子]/Fujii Yasumasa/Nishio Yumi/Watanabe Koji/Fujiwara Michiya/Kanayama Naoki/Ohmori Hitoshi/IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells./[The Journal of Immunology]

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EID
253975
EOID
679266
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LastModified
2013年2月20日(水) 16:12:57
Operator
三木 ちひろ
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[センター長]/[徳島大学.疾患酵素学研究センター]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Magari Masaki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 西川 裕美子
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Fujii Yasumasa
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Nishio Yumi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Watanabe Koji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Fujiwara Michiya
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Kanayama Naoki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Ohmori Hitoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells.

副題 任意
要約 任意

(英) In germinal centers (GCs), B cells are selected through interaction with follicular dendritic cells bearing immune complexes and follicular helper T (Tfh) cells secreting Tfh cytokines, including IL-21. To analyze these cellular interactions, we have explored culture conditions that can simulate GC B cell selection in vitro using a mouse follicular dendritic cell line, FL-YB. FL-YB cells efficiently enhanced viability of cocultured mouse B cells in a BAFF-dependent fashion. Interestingly, we found that addition of IL-21, a major Tfh cytokine, readily induced death of B cells that were cocultured with FL-YB cells, whereas IL-21 alone sustained viability of B cells in the absence of FL-YB cells. The IL-21-induced death was dependent on a low m.w. soluble factor that was released from FL-YB cells, which was finally identified as PGE(2). Treatment of B cells with IL-21 plus PGE(2), but not either alone, resulted in enhanced expression of a proapoptotic protein Bim and the upstream transcription factor Foxo1. A PGE(2) receptor isoform, EP4, was responsible for IL-21/PGE(2)-induced B cell death. Thus, PGE(2) is an endogenous chemical mediator that can switch pleiotropic actions of IL-21 on B cells. IL-21/PGE(2)-induced B cell death was rescued if B cells were costimulated via CD40. In immunized mice, deficiency of IL-21R in B cells led to a significant decrease in the frequency of activated caspase-3-positive GC B cells concomitant with impaired affinity maturation of Abs. Taken together, results implicate a physiological role of IL-21/PGE(2)-induced B cell death in GC B cell selection.

キーワード 推奨
  1. (英) Animals
  2. アポトーシス(apoptosis)
  3. (英) B-Lymphocytes
  4. 細胞分化(cell differentiation)
  5. (英) Cell Line
  6. 細胞分離(cell separation)
  7. (英) Coculture Techniques
  8. (英) Dendritic Cells, Follicular
  9. (英) Dinoprostone
  10. (英) Flow Cytometry
  11. (英) Germinal Center
  12. (英) Interleukins
  13. (英) Lymphocyte Activation
  14. (英) Male
  15. (英) Mice
  16. (英) Mice, Inbred BALB C
  17. (英) Mice, Inbred C57BL
  18. (英) Reverse Transcriptase Polymerase Chain Reaction
発行所 推奨
誌名 必須 The Journal of Immunology([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)
ISSN 任意 1550-6606
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 187
必須 8
必須 4210 4218
都市 任意
年月日 必須 2011年 9月 12日
URL 任意
DOI 任意 10.4049/jimmunol.1100934    (→Scopusで検索)
PMID 任意 21911600    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Kita-ku, Okayama 700-8530, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't