徳島大学 教育・研究者情報データベース(EDB)

1. (英) Kuwahara Tomomi

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2. (英) Ogura Yositoshi

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3. (英) Oshima Kenshiro

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4. (英) Kurokawa Ken

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5. (英) Ooka Tadasuke

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6. (英) Hirakawa Hideki

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7. (英) Itoh Takehiko

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8. (英) Nakayama-Imaohji Haruyuki

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9. (英) Ichimura Minoru

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10. (英) Itoh Kikuji

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11. 石舟 智恵子([徳島大学.大学院医歯薬学研究部.医学域.先端医学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])

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12. 前川 洋一

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13. 安友 康二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])

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14. (英) Hattori Masahira

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15. (英) Hayashi Tetsuya

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(英) The lifestyle of the segmented filamentous bacterium: a non-culturable gut-associated immunostimulating microbe inferred by whole-genome sequencing.

(英) Numerous microbes inhabit the mammalian intestinal track and strongly impact host physiology; however, our understanding of this ecosystem remains limited owing to the high complexity of the microbial community and the presence of numerous non-culturable microbes. Segmented filamentous bacteria (SFBs), which are clostridia-related Gram-positive bacteria, are among such non-culturable populations and are well known for their unique morphology and tight attachment to intestinal epithelial cells. Recent studies have revealed that SFBs play crucial roles in the post-natal maturation of gut immune function, especially the induction of Th17 lymphocytes. Here, we report the complete genome sequence of mouse SFBs. The genome, which comprises a single circular chromosome of 1 620 005 bp, lacks genes for the biosynthesis of almost all amino acids, vitamins/cofactors and nucleotides, but contains a full set of genes for sporulation/germination and, unexpectedly, for chemotaxis/flagella-based motility. These findings suggest a triphasic lifestyle of the SFB, which comprises two types of vegetative (swimming and epicellular parasitic) phases and a dormant (spore) phase. Furthermore, SFBs encode four types of flagellin, three of which are recognized by Toll-like receptor 5 and could elicit the innate immune response. Our results reveal the non-culturability, lifestyle and immunostimulation mechanisms of SFBs and provide a genetic basis for the future development of the SFB cultivation and gene-manipulation techniques.

1. (英) Animals
2. (英) Bacteria
3. (英) Biosynthetic Pathways
4. (英) Chemotaxis
5. (英) Chromosomes, Bacterial
6. (英) Flagella
7. (英) Flagellin
8. (英) Gene Order
9. (英) Genome, Bacterial
10. (英) Humans
11. (英) Intestines
12. (英) Male
13. (英) Mice
14. (英) Mice, Inbred BALB C
15. (英) Phylogeny
16. (英) Sequence Analysis, DNA
17. (英) Spores

1. (英) Article.ELocationID: 10.1093/dnares/dsr022

2. (英) Article.Affiliation: Department of Microbiology, Kagawa University, Miki-cho, Kita-gun, Kagawa, Japan.

3. (英) Article.PublicationTypeList.PublicationType: Journal Article

4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

5. (英) OtherID: PMC3158464