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著作: [富岡 重正]/Nagahama Tatsumi/[徳山 麗子]/[舘原 誠晃]/[里村 一人]/Nerve growth factor increases electrical activity of neural cells derived from murine bone marrow stromal cells/[Neuroendocrinology Letters]

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EID
252642
EOID
698433
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0
LastModified
2013年8月1日(木) 20:26:05
Operator
大家 隆弘
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Owner
富岡 重正
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 富岡 重正
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Nagahama Tatsumi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 徳山 麗子
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 舘原 誠晃
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 里村 一人
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Nerve growth factor increases electrical activity of neural cells derived from murine bone marrow stromal cells

副題 任意
要約 任意

(英) Nerve growth factor (NGF) triggers long-term neuronal excitability. We examined its effect on murine bone marrow stromal cells (BMSC)-derived neurons. With an optimal differentiation protocol, BMSCs were differentiated into neurons in culture. To confirm the probability of differentiation of BMSC into neuron, the expression of neuronal marker protein, neurofilament, was examined by immunocytochemistry. To examine the electrophysiological properties of BMSC-derived neurons, the field potentials were recorded either from nontreated (control) BMSC-derived neurons or from BMSC-derived neurons after the treatment with NGF by using extracellular recording techniques. Most BMSC-derived neurons showed spontaneous discharges whose amplitudes were up to 2 mV. When NGF at a concentration of 100 ng/ml was applied to BMSC-derived neurons, the amplitudes of discrete field potentials were gradually enlarged within 1 min after NGF application and peaked 3 min later (20-fold the size of control). However, the enlargement of the amplitudes of field potentials almost disappeared 5 min after NGF application. This finding indicates that neuronal cells derived from murine BMSCs generate discrete field potential activities spontaneously and that NGF has the effect of enlarging transient, but not sustained, electrical activity of BMSC-derived neurons.

キーワード 推奨
  1. (英) Action Potentials
  2. (英) Animals
  3. (英) Bone Marrow Cells
  4. (英) Cell Differentiation
  5. (英) Cells, Cultured
  6. (英) Male
  7. (英) Mice
  8. (英) Mice, Inbred C57BL
  9. (英) Nerve Growth Factor
  10. (英) Neurofilament Proteins
  11. (英) Neurons
  12. (英) Stromal Cells
発行所 推奨
誌名 必須 Neuroendocrinology Letters([The Society of Integrated Sciences])
(pISSN: 0172-780X)
ISSN 任意 0172-780X
ISSN: 0172-780X (pISSN: 0172-780X, eISSN: 2354-4716)
Title: Neuro endocrinology letters
Title(ISO): Neuro Endocrinol Lett
Publisher: Maghira and Maas Publications
 (NLM Catalog  (Scopus (Scopus information is found. [need login])
必須 33
必須 2
必須 177 182
都市 任意
年月日 必須 2012年 5月 初日
URL 任意
DOI 任意
PMID 任意 22592198    (→Scopusで検索)
NAID 任意
WOS 任意 000305037700011
Scopus 任意
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被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Dental Anesthesiology, The University of Tokushima Graduate School, Kuramoto-cho, Tokushima City, Tokushima, Japan. tomioka@dent.tokushima-u.ac.jp

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't