徳島大学 教育・研究者情報データベース(EDB)

1. (英) Iwahashi Shuichi

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2. 石橋 広樹([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学]/[徳島大学.病院.診療科.外科.消化器・移植外科])

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3. 宇都宮 徹

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4. 森根 裕二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.消化器・移植外科学]/[徳島大学.病院.診療科.外科.消化器・移植外科])

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5. (英) Ochir Lkhaguva Tovuu

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6. 花岡 潤

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7. 森 大樹([徳島大学.病院.診療科.外科.小児外科・小児内視鏡外科])

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8. 池本 哲也([徳島大学.病院.中央診療施設等.安全管理部]/[徳島大学.病院.診療科.外科.消化器・移植外科])

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9. 居村 暁

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10. 島田 光生

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(英) Effect of histone deacetylase inhibitor in combination with 5-fluorouracil on pancreas cancer and cholangiocarcinoma cell lines.

(英) Histone deacetylase (HDAC) is well known to be associated with tumorigenesis through epigenetic regulation, and its inhibitors (HDACIs) induce differentiation and apoptosis of tumor cells. We examined the therapeutic effects of valproic acid (VPA, a HDACI) with a combination of 5-fluorouracil (5-FU) in vitro. A human pancreas cancer cell line (SUIT-2) and a cholangiocarcinoma cell line (HuCCT1) were used. Cell viabilities were evaluated by a cell proliferation assay. We determined the anticancer effects of VPA combined with 5-FU in these cell lines. Pancreas cancer (SUIT-2): No effect of 5-FU (1.0 µM) was observed, but 17% and 30% of proliferation-inhibitory effects were recognized in a dose of 2.5 or 5.0 µM, respectively. Cell viability was only weakly reduced by VPA (0.5 mM). However, in combination of 5-FU (1.0 µM) with VPA (0.5 mM), 19% of inhibitory effect was observed. Cholangiocarcinoma (HuCCT1): 5-FU (1.0 µM) did not suppress the cell viability, but 5-FU (2.5 µM) suppressed by 23%. VPA (0.5 mM) did not suppress the cell viability, while VPA (1.0 mM) weakly decreased it by 11%. Combination of 5-FU (1.0 µM) and VPA (0.5 mM) markedly reduced the cell viability by 30%. VPA augmented the anti-tumor effects of 5-FU in cancer cell lines. Therefore, a combination therapy of 5-FU plus VPA may be a promising therapeutic option for patients with pancreas cancer and cholangiocarcinoma.

1. (英) Antineoplastic Combined Chemotherapy Protocols
2. (英) Bile Duct Neoplasms
3. (英) Bile Ducts, Intrahepatic
4. (英) Cell Line, Tumor
5. (英) Cell Proliferation
6. (英) Cell Survival
7. (英) Cholangiocarcinoma
8. (英) Fluorouracil
9. (英) Histone Deacetylase Inhibitors
10. (英) Humans
11. (英) Pancreatic Neoplasms
12. (英) Valproic Acid
13. (日) 欧文論文

1. (英) Article.Affiliation: Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't