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著作: Ikedo D/[大石 慶二]/Yamauchi N/[片岡 正俊]/[木戸 淳一]/[永田 俊彦]/Stimulatory effects of phenytoin on osteoblastic differentiation of fetal rat calvaria cells in culture./[Bone]

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EID
250710
EOID
849766
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0
LastModified
2017年3月8日(水) 16:08:01
Operator
三木 ちひろ
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TRUE
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Owner
木戸 淳一
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Ikedo D
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 大石 慶二
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Yamauchi N
    データベース中に適合する可能性のある以下の情報を発見しました
    [個人] 山内 健
    [個人] 山内 康伸
    この情報が上記に掲げた個人の業績等に分類されるためには参照登録が必要です.上記に掲げた個人本人に該当する場合には参照登録に変更してください.
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 片岡 正俊
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 木戸 淳一([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 永田 俊彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Stimulatory effects of phenytoin on osteoblastic differentiation of fetal rat calvaria cells in culture.

副題 任意
要約 任意

(英) Phenytoin (diphenylhydantoin, DPH), an anticonvulsant drug for epileptic patients, has several adverse effects, including calvarial thickening and coarsening of the facial features, which occur with chronic DPH therapy. While previous studies have demonstrated that DPH has an anabolic action on bone cells in vivo and in vitro, the basis of these effects is not fully understood. In this study, the effect of DPH on osteoblastic differentiation of fetal rat calvaria (RC) cells in culture was investigated by measuring bone nodule (BN) formation, cell growth, alkaline phosphatase (ALPase) activity, collagen synthesis, and expression of osteocalcin (OC) and osteopontin (OP) mRNAs. Continuous treatment of RC cells with DPH for 18 days dose-dependently increased the mineralized BN number by 1.2-1.7-fold at concentrations of 12.5-200 micromol/L DPH. Cell growth was not affected at the same concentrations of DPH. ALPase activity was stimulated by DPH (1.1-1.9-fold) dose-dependently and was maintained at higher levels in DPH-treated cells throughout the experimental period. DPH increased mineralized and unmineralized BN formations both in the presence and the absence of 10(-8) mol/L dexamethasone (Dex). Expression of OC and OP mRNAs was markedly augmented by DPH on days 12-24 and on days 12-18, respectively. While control mRNA levels of OC and OP increased with time, the increases in DPH-treated cells were greater than those of the controls and the stimulatory effects were dose-dependent. Type I collagen was also influenced by DPH; mRNA level was enhanced and the percentage of collagen synthesized was increased significantly, by 200 micromol/L DPH. When DPH was added in three different culture stages, days 1-6 (growth), days 7-12 (matrix development), and days 13-18 (mineralization), BN formation was influenced primarily on days 1-6 and secondarily on days 7-12, but not on days 13-18, suggesting that DPH increased BN formation by enhancing not only the proportion of osteoprogenitor cells in the early stage but also the proportion of functional osteoblasts in the middle stage within mixed-cell populations. Moreover, such increases were detected in conditions of both Dex(+) and Dex(-). These findings demonstrate that DPH stimulates osteoblast-associated markers such as BNs, ALPase, OC, OP, and type I collagen by continuously affecting the stages of growth and matrix development in RC cells, and suggests that the stimulatory effects by DPH may possibly be induced independent of those by Dex.

キーワード 推奨
  1. (英) Alkaline Phosphatase
  2. (英) Animals
  3. (英) Anticonvulsants
  4. (英) Calcification, Physiologic
  5. 細胞分化(cell differentiation)
  6. 細胞分裂(cell division)
  7. (英) Cells, Cultured
  8. コラーゲン(collagen)
  9. (英) Dexamethasone
  10. (英) Fetus
  11. (英) Osteoblasts
  12. (英) Osteocalcin
  13. オステオポンティン(osteopontin)
  14. (英) Phenytoin
  15. (英) RNA, Messenger
  16. (英) Rats
  17. (英) Sialoglycoproteins
  18. (英) Skull
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] 頭蓋底手術 (skull base surgery)
  19. (英) Stem Cells
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] 人工多能性幹細胞 (induced pluripotent stem cells)
発行所 推奨
誌名 必須 Bone(International Bone and Mineral Society)
(pISSN: 8756-3282, eISSN: 1873-2763)
ISSN 任意 8756-3282
ISSN: 8756-3282 (pISSN: 8756-3282, eISSN: 1873-2763)
Title: Bone
Title(ISO): Bone
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 25
必須 6
必須 653 660
都市 任意
年月日 必須 1999年 12月 初日
URL 任意
DOI 任意
PMID 任意 10593409    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Periodontology and Endodontology, Tokushima University School of Dentistry, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article