徳島大学 教育・研究者情報データベース(EDB)

1. 木戸 淳一

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2. (英) Yamauchi N

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3. 大石 慶二

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4. 片岡 正俊

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5. 西川 聖二(西川歯科クリニック)

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6. (英) Nakamura T

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7. (英) Kadono H

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8. (英) Ikedo D

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9. 上野 明道

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10. (英) Nonomura N

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11. (英) Okuyama A

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12. 永田 俊彦

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(英) Inhibition of osteoblastic cell differentiation by conditioned medium derived from the human prostatic cancer cell line PC-3 in vitro.

(英) Human prostatic carcinoma frequently metastasizes to bone tissue and activates bone metabolism, especially bone formation, at the site of metastasis. It has been reported that an extract of prostatic carcinoma and conditioned medium (CM) of a human prostatic carcinoma cell line, PC-3, established from a bone metastastic lesion, stimulate osteoblastic cell proliferation. However, there is little information about the effect of PC-3 CM on the differentiation of osteoblastic cells. In this study, we investigated the effect of PC-3 CM on the differentiation of two types of osteoblastic cells, primary fetal rat calvaria (RC) cells containing many undifferentiated osteoprogenitor cells, and ROS 17/2.8, a well-differentiated rat osteosarcoma cell line. PC-3 CM inhibited bone nodule formation and the activity of alkaline phosphatase (ALPase), an osteoblastic marker enzyme, on days 7, 14, and 21 (RC cells) or 3, 6, and 9 (ROS 17/2.8 cells) in a dose-dependent manner (5-30% CM). However, the CM did not affect cell proliferation or cell viability. PC-3 CM was found to markedly block the gene expression of ALPase and osteocalcin (OCN) mRNAs but had no effect on the mRNA expression of osteopontin (OPN), the latter two being noncollagenous proteins related to bone matrix mineralization. These findings suggest that PC-3 CM contains a factor that inhibits osteoblastic cell differentiation and that this factor may be involved in the process of bone metastasis from prostatic carcinoma.

1. (英) Alkaline Phosphatase
2. (英) Animals
3. (英) Bone and Bones
4. 細胞分化(cell differentiation)
5. 細胞分裂(cell division)
6. (英) Culture Media, Conditioned
7. (英) Embryo, Mammalian
8. 遺伝子発現(gene expression)
9. (英) Humans
10. (英) Male
11. (英) Osteoblasts
12. (英) Osteocalcin
13. オステオポンティン(osteopontin)
14. (英) Osteosarcoma
15. (英) Prostatic Neoplasms
16. (英) RNA, Messenger
17. (英) Rats
18. (英) Sialoglycoproteins
19. (英) Time Factors
20. (英) Tumor Cells, Cultured

1. (英) Article.Affiliation: Department of Periodontology and Endodontology, Tokushima University School of Dentistry, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article