徳島大学 教育・研究者情報データベース(EDB)

1. 大石 慶二

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2. (英) Ishida H

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3. 永田 俊彦

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4. (英) Yamauchi N

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5. (英) Tsurumi C

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6. 西川 聖二(西川歯科クリニック)

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7. (英) Wakano Y

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(英) Thyroid hormone suppresses the differentiation of osteoprogenitor cells to osteoblasts, but enhances functional activities of mature osteoblasts in cultured rat calvaria cells.

(英) The effects of thyroid hormone on osteoblastic differentiation and activity were studied in fetal rat calvaria (RC) cells cultured for up to 30 days in medium supplemented with thyroid hormone-depleted serum. In this condition, the cells proliferated and differentiated to form mineralized bone nodules (BN) and expressed osteoblastic markers such as alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN). The continuous presence of triiodothyronine (T3) at 10(-9)-10(-8) M in the medium inhibited the osteoblastic differentiation: 34% decrease in ALP activity on day 12 and 60% decrease in BN formation on day 15 at 10(-8) M. T3 at these doses had no effect on the DNA content of RC cells at confluence (day 6). Short-term (48-h) exposure of T3 at 10(-9) M or higher decreased ALP activity when RC cells were differentiating (days 7-11). However, when BN formation by the cells had already reached a plateau (day 28), the activity was increased by treatment with T3 at 10(-7)-10(-6) M. OCN production was increased dose dependently by this treatment with T3 (2.1-fold and 1.3-fold of control at 10(-8) M on days 11 and 28, respectively). Similar increases were observed in the levels of OCN mRNA. In addition, increases in phosphorylated OPN in the medium (day 11) and mineralized matrix (day 28) were observed (1.5-fold at 10(-8)-10(-6) M), while OPN synthesis and the level of its mRNA were depressed by T3 (60-70% of control at 10(-8) M). These results suggest that T3 regulates osteoblastic differentiation and activity depending on the state of cell differentiation: T3 suppresses the differentiation of osteoprogenitor cells to osteoblasts, but enhances the functional activity of mature osteoblasts.

1. (英) Alkaline Phosphatase
2. (英) Animals
3. (英) Bone Development
4. 細胞分化(cell differentiation)
5. 細胞分裂(cell division)
6. (英) Cells, Cultured
7. 遺伝子発現(gene expression)
8. (英) Osteoblasts
9. (英) Osteocalcin
10. オステオポンティン(osteopontin)
11. (英) RNA, Messenger
12. (英) Rats
13. (英) Sialoglycoproteins
14. (英) Skull
15. (英) Time Factors
16. (英) Triiodothyronine

1. (英) Article.Affiliation: Department of Periodontology and Endodontology, Tokushima University School of Dentistry, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article