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著作: [藤原 広明]/Ueta Yoichi/Suzuki Hitoshi/Katoh Akiko/Ohbuchi Toyoaki/Otsubo Hiroki/Dayanithi Govindan/Murphy David/Robust up-regulation of nuclear red fluorescent-tagged fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double-transgenic rat./[Endocrinology]

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EID
248741
EOID
726697
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0
LastModified
2014年3月4日(火) 17:08:22
Operator
三木 ちひろ
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TRUE
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Owner
[学科長]/[徳島大学.医学部.医学科]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 藤原 広明
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Ueta Yoichi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Suzuki Hitoshi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Katoh Akiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Ohbuchi Toyoaki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Otsubo Hiroki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Dayanithi Govindan
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Murphy David
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Robust up-regulation of nuclear red fluorescent-tagged fos marks neuronal activation in green fluorescent vasopressin neurons after osmotic stimulation in a double-transgenic rat.

副題 任意
要約 任意

(英) The up-regulation in the expression of mRNA or protein encoded by the c-fos gene is widely used as a marker of neuronal activation elicited by various stimuli. To facilitate the detection of activated neurons, we generated transgenic rats expressing a fusion gene consisting of c-fos coding sequences in frame with monomeric red fluorescent protein 1 (mRFP1) under the control of c-fos gene regulatory sequences (c-fos-mRFP1 rats). In c-fos-mRFP1 transgenic rats, 90 min after hypertonic saline ip administration, nuclear mRFP1 fluorescence was observed abundantly in brain regions known to be osmosensitive, namely the median preoptic nucleus, organum vasculosum lamina terminalis, supraoptic nucleus, paraventricular nucleus, and subfornical organ. Immunohistochemistry for Fos protein confirmed that the distribution of Fos-like immunoreactivity in nontransgenic rats was similar to those of mRFP1 fluorescence after ip administration of hypertonic saline in the transgenic rats. Several double-transgenic rats were obtained from matings between transgenic rats expressing an arginine vasopressin-enhanced green fluorescent protein fusion gene (AVP-eGFP rats) and c-fos-mRFP1 rats. In these double-transgenic rats, almost all eGFP neurons in the supraoptic nucleus and PVN expressed nuclear mRFP1 fluorescence 90 min after hypertonic saline administration. The c-fos-mRFP1 rats are a powerful tool that enables the facile identification of activated neurons in the nervous system. Furthermore, when combined with transgenes expressing another fluorophore under the control of cell-specific regulatory sequences, activation of specific neuronal cell types in response to physiological cues can be readily detected.

キーワード 推奨
  1. (英) Animals
  2. (英) Arginine Vasopressin
  3. (brain)
  4. (英) Female
  5. (英) Green Fluorescent Proteins
  6. 視床下部(hypothalamus)
  7. 免疫組織化学(immunohistochemistry)
  8. (英) Luminescent Proteins
  9. (英) Male
  10. (英) Mice
  11. (英) Mice, Inbred C57BL
  12. (英) Microscopy, Confocal
  13. (英) Microscopy, Fluorescence
  14. (英) Neurons
  15. (英) Paraventricular Hypothalamic Nucleus
  16. (英) Proto-Oncogene Proteins c-fos
  17. (英) Rats
  18. (英) Rats, Transgenic
  19. (英) Rats, Wistar
  20. (英) Recombinant Fusion Proteins
  21. (英) Regulatory Sequences, Nucleic Acid
  22. (英) Saline Solution, Hypertonic
  23. (英) Subfornical Organ
  24. (英) Supraoptic Nucleus
  25. (英) Up-Regulation
発行所 推奨
誌名 必須 Endocrinology([The Endocrine Society])
(pISSN: 0013-7227, eISSN: 1945-7170)
ISSN 任意 1945-7170
ISSN: 0013-7227 (pISSN: 0013-7227, eISSN: 1945-7170)
Title: Endocrinology
Title(ISO): Endocrinology
Publisher: Endocrine Society
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 150
必須 12
必須 5633 5638
都市 任意
年月日 必須 2009年 10月 22日
URL 任意
DOI 任意 10.1210/en.2009-0796    (→Scopusで検索)
PMID 任意 19850746    (→Scopusで検索)
NAID 任意
WOS 任意 000272042300048
Scopus 任意 2-s2.0-71949097426
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't