徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [岩田 武男]/[谷口 寿章]/[桒島 正道]/[谷口 貴子]/Yuko Okuda/[助野 晃子]/[石本 恭子]/[水澤 典子]/[吉本 勝彦]/The action of D-dopachrome tautomerase as an adipokine in adipocyte lipid metabolism/[PLoS ONE]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
248470
EOID
878057
Map
0
LastModified
2017年12月4日(月) 14:03:36
Operator
[ADMIN]
Avail
TRUE
Censor
0
Owner
岩田 武男
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 岩田 武男
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 谷口 寿章
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 桒島 正道
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 谷口 貴子
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Yuko Okuda
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 助野 晃子([徳島大学.大学院ヘルスバイオサイエンス研究部.学長裁量プロジェクト]/->個人[原田 晃子])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 石本 恭子
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. 水澤 典子([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  9. 吉本 勝彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) The action of D-dopachrome tautomerase as an adipokine in adipocyte lipid metabolism

副題 任意
要約 任意

(英) Adipose tissue is a critical exchange center for complex energy transactions involving triacylglycerol storage and release. It also has an active endocrine role, releasing various adipose-derived cytokines (adipokines) that participate in complex pathways to maintain metabolic and vascular health. Here, we found D-dopachrome tautomerase (DDT) as an adipokine secreted from human adipocytes by a proteomic approach. DDT mRNA levels in human adipocytes were negatively correlated with obesity-related clinical parameters such as BMI, and visceral and subcutaneous fat areas. Experiments using SGBS cells, a human preadipocyte cell line, revealed that DDT mRNA levels were increased in an adipocyte differentiation-dependent manner and DDT was secreted from adipocytes. In DDT knockdown adipocytes differentiated from SGBS cells that were infected with the adenovirus expressing shRNA against the DDT gene, mRNA levels of genes involved in both lipolysis and lipogenesis were slightly but significantly increased. Furthermore, we investigated AMP-activated protein kinase (AMPK) signaling, which phosphorylates and inactivates enzymes involved in lipid metabolism, including hormone-sensitive lipase (HSL) and acetyl-CoA carboxylase (ACC), in DDT knockdown adipocytes. The AMPK phosphorylation of HSL Ser-565 and ACC Ser-79 was inhibited in DDT knockdown cells and recovered in the cells treated with recombinant DDT (rDDT), suggesting that down-regulated DDT in adipocytes brings about a state of active lipid metabolism. Furthermore, administration of rDDT in db/db mice improved glucose intolerance and decreased serum free fatty acids levels. In the adipose tissue from rDDT-treated db/db mice, not only increased levels of HSL phosphorylated by AMPK, but also decreased levels of HSL phosphorylated by protein kinase A (PKA), which phosphorylates HSL to promote its activity, were observed. These results suggested that DDT acts on adipocytes to regulate lipid metabolism through AMPK and/or PKA pathway(s) and improves glucose intolerance caused by obesity.

キーワード 推奨
  1. (英) AMP-Activated Protein Kinases
  2. (英) Adipocytes
  3. (英) Adipokines
  4. (英) Animals
  5. (英) Blotting, Western
  6. (英) Body Mass Index
  7. (英) Cell Differentiation
  8. (英) DNA Primers
  9. (英) Gene Knockdown Techniques
  10. (英) Humans
  11. (英) Intramolecular Oxidoreductases
  12. (英) Lipid Metabolism
  13. (英) Mice
  14. (英) Microscopy, Fluorescence
  15. (英) Phosphorylation
  16. (英) Proteomics
  17. (英) Reverse Transcriptase Polymerase Chain Reaction
  18. (英) Signal Transduction
発行所 推奨
誌名 必須 PLoS ONE(Public Library of Science)
(eISSN: 1932-6203)
ISSN 任意 1932-6203
ISSN: 1932-6203 (eISSN: 1932-6203)
Title: PloS one
Title(ISO): PLoS One
Publisher: Public Library of Science
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 7
必須 3
必須 e33402 e33402
都市 任意
年月日 必須 2012年 3月 12日
URL 任意
DOI 任意 10.1371/journal.pone.0033402    (→Scopusで検索)
PMID 任意 22428043    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
機関リポジトリ 109634
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  4. (英) OtherID: PMC3299789