徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.総合科学部.社会創生学科.環境共生コース(2009年4月1日〜2024年3月31日)
2. 徳島大学.大学院ソシオ・アーツ・アンド・サイエンス研究部.創生科学研究部門.環境共生科学分野(2009年4月1日〜2016年3月31日)

1. (英) Kanbara Yasuhiro / (日) 上原 靖弘 / (読) かんばら やすひろ

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2. (英) Seo Hakaru

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3. (英) Nishimura Yumiko

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4. 小山 保夫

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(英) Decrease in intracellular Zn2+ level by propranolol: A model experiment using rat thymocytes

(英) Propranolol is a representative β-blocker used for the treatment of cardiovascular diseases. Because the use of propranolol expands for some clinical purposes that are not related to its β-blocking action, it is necessary to further examine cellular actions of propranolol. We revealed that propranolol decreased the intracellular Zn2+ level in rat thymocytes by the use of cytometric technique with FluoZin-3, a fluorescent indicator of intracellular Zn2+. Propranolol decreased the influx of extracellular Zn2+. However, the agent decreased the intracellular Zn2+ level even in the presence of DTPA, a chelator of extracellular Zn2+. Thus, the decrease in intracellular Zn2+ level by propranolol was not due to the decrease in Zn2+ influx by propranolol. Propranolol increased the cellular content of nonprotein thiol that was estimated by the use of 5-chloromethylfluorescein fluorescence, an indicator for non-proteinous thiol. Since non-proteinous thiols can make a complex with Zn2+, propranolol may increase the cellular content of nonprotein thiol, resulting in the decrease in intracellular Zn2+ level. Since the concentrations of propranolol to affect both intracellular Zn2+ level and cellular content of nonprotein thiol are higher than those reported under clinical conditions, it is difficult to emphasize clinical implications from present results.

(日) Propranolol is a representative β-blocker used for the treatment of cardiovascular diseases. Because the use of propranolol expands for some clinical purposes that are not related to its β-blocking action, it is necessary to further examine cellular actions of propranolol. We revealed that propranolol decreased the intracellular Zn2+ level in rat thymocytes by the use of cytometric technique with FluoZin-3, a fluorescent indicator of intracellular Zn2+. Propranolol decreased the influx of extracellular Zn2+. However, the agent decreased the intracellular Zn2+ level even in the presence of DTPA, a chelator of extracellular Zn2+. Thus, the decrease in intracellular Zn2+ level by propranolol was not due to the decrease in Zn2+ influx by propranolol. Propranolol increased the cellular content of nonprotein thiol that was estimated by the use of 5-chloromethylfluorescein fluorescence, an indicator for non-proteinous thiol. Since non-proteinous thiols can make a complex with Zn2+, propranolol may increase the cellular content of nonprotein thiol, resulting in the decrease in intracellular Zn2+ level. Since the concentrations of propranolol to affect both intracellular Zn2+ level and cellular content of nonprotein thiol are higher than those reported under clinical conditions, it is difficult to emphasize clinical implications from present results.

1. (英) Propranolol
2. (日) Intracellular Zn²⁺level
3. (英) FluoZin-3