徳島大学 教育・研究者情報データベース(EDB)

1. 安藝 健作([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.医用検査学系.細胞・免疫解析学])

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2. (日) 相原 美奈子

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3. (日) 森内 貴子

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4. (日) 森 美和

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5. 細井 英司

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(英) Preparation method for a specific anti-H lectin isolated from goya seeds and its application in clinical laboratories

(日) ゴーヤ種子抽出抗Hレクチンの臨床検査への応用

(日) After the discovery of the phenomenon of aggregation of red blood cells with castor beanextract by Peter Hermann Stillmark in1888, various plant hemagglutinins were discovered fromleguminous plant seed extracts. These plant hemagglutinins were called"lectins"by William C.Boyd et al . Lectins are sugar-binding proteins that are highly specific for their sugar moieties,physiological activity, and hemagglutinating activity. In particular, lectins displaying blood-groupspecificity are important for blood-group typing and antigen recognition. In recent years, Ulexlectin(anti-H lectin)extracted from the Ulex europaeus seed was used to subdivide blood of typeO, and the subgroup of ABO, but Ulex europaeus is now difficult to obtain, so it is necessary to buyexpensive reagents.On the other hand, Momordica charantias (goya)seed extract(goya lectin)was recentlyrevealed to show higher anti-H activity than Ulex lectin. Goya is readily available and inexpensiveto obtain. Therefore, we examined a simple method for the preparation of goya lectin.As a result, we were able to establish a simple method for preparing goya lectin isolated fromgoya seeds, and confirm its stability against long-term storage and temperature and usefulness asan anti-H lectin. We expect this method to be used in clinical practice in the future.

1. (英) momordica charantias (goya) seeds
2. (英) plant hemagglutinins
3. (英) anti-H lectin
4. (英) ABO blood typing
5. (英) clinical laboratories