徳島大学 教育・研究者情報データベース(EDB)

1. 安藝 健作([徳島大学.大学院医歯薬学研究部.保健学域.保健科学部門.医用検査学系.細胞・免疫解析学])

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2. (英) Izumi Azusa / (日) 泉 あずさ / (読) いずみ あずさ

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3. 細井 英司

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(英) The evaluation of histo-blood group ABO typing by flow cytometric and PCR Amplification of Specific Alleles analyses and their application in clinical laboratories

(英) ABO antigens are oligosaccharide antigens, and are widely distributed on red blood and tissue cells as well as in saliva and body fluid. Therefore, these antigens are important not only for blood transfusion, but also for tissue cell and organ transplantations. Also, blood, hair, and seminal fluid are important sources of evidence at crime scenes, and these antigens are some of the most important markers for personal identification in forensic investigations. Here, we describe the development and use of quantitative analysis of A, B, and H antigens on red blood cells by employing flow cytometric analysis and the ABO genotyping method based on PCR-amplification of specific alleles (PASA) within DNA, especially from blood and saliva. In this study, flow cytometric analysis could be used to compare the differences between the expression of A and/or B and H antigens on red blood cells with various phenotypes, and the PASA method was able to determine the genotype of the type cisA(2)B(3) pedigree using only DNA extracted from saliva. These analysis methods are simple and useful for judging the ABO blood group system and genotyping, and are used widely throughout research and clinical laboratories and forensic fields.

1. (英) ABO Blood-Group System
2. (英) Alleles
3. (英) Antigens, Bacterial
4. (英) Biometric Identification
5. (英) Flow Cytometry
6. (英) Forensic Pathology
7. (英) Histocompatibility Testing
8. (英) Humans
9. (英) Polymerase Chain Reaction

1. (英) Article.Affiliation: Subdivision of Biomedical Laboratory Sciences, Graduate School of Health Sciences, the University of Tokushima, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article